Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Taurocholic acid-induced secretion in normal and cystic fibrosis mouse ileum
Autore:
Hardcastle, J; Hardcastle, PT; Chapman, J; Taylor, CJ;
Indirizzi:
Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TH, S Yorkshire, England Univ Sheffield Sheffield S Yorkshire England S10 2TH S Yorkshire, England Univ Sheffield, Div Child Hlth, Sheffield S10 2TN, S Yorkshire, England Univ Sheffield Sheffield S Yorkshire England S10 2TN S Yorkshire, England
Titolo Testata:
JOURNAL OF PHARMACY AND PHARMACOLOGY
fascicolo: 5, volume: 53, anno: 2001,
pagine: 711 - 719
SICI:
0022-3573(200105)53:5<711:TASINA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION-TRANSPORT; SMALL-INTESTINE; MAST-CELLS; IN-VITRO; COLON; HISTAMINE; CONTRIBUTE; FAILURE; MUCOSA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Hardcastle, J Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TH, S Yorkshire, England Univ Sheffield Sheffield S Yorkshire England S10 2TH ngland
Citazione:
J. Hardcastle et al., "Taurocholic acid-induced secretion in normal and cystic fibrosis mouse ileum", J PHARM PHA, 53(5), 2001, pp. 711-719

Abstract

Bile acids cause secretion throughout the intestinal tract and this process contributes to maintaining the fluidity of intestinal contents. In cysticfibrosis (CF) defective intestinal secretion can lead to excessive dehydration of the luminal contents and the development of clinical symptoms. Thisstudy was designed to investigate bile acid-induced secretion in mouse ileum and to determine whether this process was defective in CF. Taurocholic acid-induced secretion was monitored as a rise in short-circuit current (SCC) in ileal sheets from normal (Swiss MF1) and transgenic CF mice. Taurocholic acid increased the SCC in both intact and stripped ileal sheets from Swiss MF1 mice. This effect was due to a stimulation of electrogenic Cl- secretion as it was inhibited by Cl--free conditions, serosal furosemide (frusemide), mucosal diphenyl amine-2-carboxylic acid (DPC) and increased serosal K+ concentration, without being affected by reduced mucosal Na+ concentration. Taurocholic acid-induced secretion was inhibited by tetrodotoxin. indicating the involvement of a neural pathway, but this did not include capsaicin-sensitive afferent neurons or muscarinic: cholinoreceptors. Mucosal mastcells also contributed to the response. Responses in tissues from transgenic wild-type mice were similar to those obtained with Swiss MF1 animals, but ilea from CF mice exhibited a lower basal SCC with significantly reduced secretory responses to acetylcholine and taurocholic acid. We concluded that taurocholic acid induces ileal secretion by a mechanism that entails activation of enteric: nerves and degranulation of mucosal mast cells. Impairedbile acid-induced secretion in CF may contribute to luminal dehydration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:30:54