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Titolo:
iNOS and nitrotyrosine expression after spinal cord injury
Autore:
Xu, J; Kim, GM; Chen, SW; Yan, P; Ahmed, SH; Ku, G; Beckman, JS; Xu, XM; Hsu, CY;
Indirizzi:
Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Dept Neurol, St Louis, MO 63110 USA Washington Univ, Sch Med, Ctr Study Nervous Syst Injury, St Louis, MO 63110 USA Washington Univ St Louis MO USA 63110 Syst Injury, St Louis, MO 63110 USA St Louis Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63104 USA St Louis Univ St Louis MO USA 63104 t & Neurobiol, St Louis, MO 63104 USA Univ Alabama, Sch Med, Dept Anesthesiol & Biochem, Birmingham, AL USA UnivAlabama Birmingham AL USA Anesthesiol & Biochem, Birmingham, AL USA
Titolo Testata:
JOURNAL OF NEUROTRAUMA
fascicolo: 5, volume: 18, anno: 2001,
pagine: 523 - 532
SICI:
0897-7151(200105)18:5<523:IANEAS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; TRAUMATIC BRAIN INJURY; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; KAPPA-B ACTIVATION; INDUCIBLE FORM; CELL-DEATH; RATS; MICE; METHYLPREDNISOLONE;
Keywords:
cytokines; inflammation; nitric oxide; oxygen free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Hsu, CY Washington Univ, Sch Med, Dept Neurol, Box 8111,660 S Euclid Ave, St Louis, MO 63110 USA Washington Univ Box 8111,660 S Euclid Ave St Louis MO USA 63110 SA
Citazione:
J. Xu et al., "iNOS and nitrotyrosine expression after spinal cord injury", J NEUROTRAU, 18(5), 2001, pp. 523-532

Abstract

Secondary tissue damage after spinal cord injury (SCI) may be due to inflammatory mediators. After SCI, the nuclear factor-kappaB (NF-kappaB) transcription factor can activate many pro-inflammatory genes, one of which is inducible nitric oxide synthase (iNOS), iNOS catalyzes the synthesis of nitricoxide (NO), a key inflammatory mediator, which in turn reacts with superoxide to generate peroxynitrite, Peroxynitrite is a strong oxidant that can damage cellular enzymes, membranes, and subcellular organelles through the nitration of tyrosine residues on proteins. The presence of nitrotyrosine (NT) is an indirect chemical indicator of toxic NO and peroxynitrite-induced cellular damage. Using a New York University (NYU) impactor to induce SCI in adult rats, we examined the temporal and cellular expression of iNOS and NT, We observed a progressive increase in;iNOS expression in the injured cord starting at day 1 with maximal expression occurring at day 7, as determined by Western blot analysis. iNOS expression corresponded temporally to anincrease in iNOS enzyme activity after SCI, In parallel with the progressive increase in iNOS activity, NT expression also increased with time after SCI, The iNOS and:NT immunoreactivity was localized in neurons, astrocytes,endothelial cells and ependymal cells at the epicenter and adjacent to theregion of spinal cord impact and injury. Results from the present study suggest that increased iNOS and peroxynitrite anion, as reflected by the progressive accumulation of NT in the injured impacted spinal cord, may contribute to the secondary injury process after SCI.

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Documento generato il 01/06/20 alle ore 02:34:52