Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Oxyhemoglobin induces caspase-mediated cell death in cerebral endothelial cells
Autore:
Meguro, T; Chen, B; Lancon, J; Zhang, JH;
Indirizzi:
Univ Mississippi, Med Ctr, Dept Neurosurg, Jackson, MS 39216 USA Univ Mississippi Jackson MS USA 39216 pt Neurosurg, Jackson, MS 39216 USA
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 4, volume: 77, anno: 2001,
pagine: 1128 - 1135
SICI:
0022-3042(200105)77:4<1128:OICCDI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL SUBARACHNOID HEMORRHAGE; ICE-LIKE PROTEASES; CEREBROSPINAL-FLUID; BARRIER DISRUPTION; FREE-RADICALS; GENE CED-3; APOPTOSIS; HEMOGLOBIN; VASOSPASM; INHIBITION;
Keywords:
apoptosis; caspases; cerebral vasospasm; endothelium; oxyhemoglobin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, JH Univ Mississippi, Med Ctr, Dept Neurosurg, 2500 N State St, Jackson, MS 39216 USA Univ Mississippi 2500 N State St Jackson MS USA 39216 39216 USA
Citazione:
T. Meguro et al., "Oxyhemoglobin induces caspase-mediated cell death in cerebral endothelial cells", J NEUROCHEM, 77(4), 2001, pp. 1128-1135

Abstract

Damaged endothelium is one of the pathological changes of the cerebral vasospastic vessels following subarachnoid hemorrhage. Our recent study shows that oxyhemoglobin (OxyHb) induces apoptosis in vascular endothelial cells. Apoptosis generally requires the action of various classes of proteases, including a family of cysteine proteases, known collectively as the caspases. This study was undertaken to investigate the activation of caspases and the efficacy of caspase inhibitors, z-IETD-fmk and z-LEHD-fmk, for oxyhemoglobin-induced apoptosis in vascular endothelial cells. Cultured bovine brainmicrovascular endothelial cells (passages 5-9) were used for this study. OxyHb (10 mu mol/L) was added during the 24-72 h incubation with and withoutcaspase-8 or -9 inhibitors (z-IETD-fmk and z-LEHD-fmk). Counting survivingcells, DNA laddering, western blotting of poly(ADP-ribose) polymerase, andmeasurement of caspase activities were employed to confirm the cytotoxic effects of OxyHb and the protective effects of the caspase inhibitors. OxyHbproduced cell detachment in a time-dependent manner and increased caspase-8 and -9 activities in the cells. z-IETD-fmk and z-LEHD-fmk (100 mu mol/L) attenuated OxyHb-induced cell loss, DNA laddering, and proteolytic cleavageof PARP, although a lower concentration (10 mu mol/L) of caspase inhibitors showed partial effects. OxyHb activates caspase-8 and -9 in cultured vascular endothelial cells, and blocking the action of the caspases with the inhibitors efficiently prevents loss of vascular endothelial cells from OxyHb-induced apoptosis in vitro. These results suggest that the caspase cascadeparticipates in OxyHb-induced apoptosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 23:14:04