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Titolo:
Quantitative analysis of chemokine expression by dendritic cell subsets invitro and in vivo
Autore:
Vissers, JLM; Hartgers, FC; Lindhout, E; Teunissen, MBM; Figdor, CG; Adema, GJ;
Indirizzi:
Univ Nijmegen, Dept Tumor Immunol, Med Ctr St Radboud, NL-6525 GA Nijmegen, Netherlands Univ Nijmegen Nijmegen Netherlands NL-6525 GA 5 GA Nijmegen, Netherlands Univ Amsterdam, Acad Med Ctr, Dept Dermatol, NL-1012 WX Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1012 WX X Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 5, volume: 69, anno: 2001,
pagine: 785 - 793
SICI:
0741-5400(200105)69:5<785:QAOCEB>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE-DERIVED CHEMOKINE; T-LYMPHOCYTES; HUMAN BLOOD; IN-VIVO; SYSTEM; PCR; RESPONSES; MATURATION; MONOCYTES; PRECURSOR;
Keywords:
human; real-time quantitative PCR; monocytes; blood DCs; Langerhans cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Adema, GJ Univ Nijmegen, Dept Tumor Immunol, Med Ctr St Radboud, Geert Grooteplein Zuid 28, NL-6525 GA Nijmegen, Netherlands Univ Nijmegen Geert Grooteplein Zuid 28 Nijmegen Netherlands NL-6525 GA
Citazione:
J.L.M. Vissers et al., "Quantitative analysis of chemokine expression by dendritic cell subsets invitro and in vivo", J LEUK BIOL, 69(5), 2001, pp. 785-793

Abstract

Upon maturation, dendritic cells (DCs) have to adjust their chemokine expression to sequentially attract different leukocyte subsets. We used real-time quantitative polymerase chain reaction analysis to study in detail the expression of 12 chemokines involved in the recruitment of leukocytes into and inside secondary lymphoid organs, by DCs in distinct differentiation stages, both in vitro and in vivo, Monocyte-derived immature DCs expressed high levels of DC chemokine 1 (DC-CK1), EBI1-Ligand chemokine (ELC), macrophage-derived chemokine (MDC), macrophage-inflammatory protein (MIP)-1 alpha, and thymus and activation-regulated chemokine (TARC), Upon maturation, DCs up-regulated the expression of DC-CK1 (60-fold), ELC (7-fold), and TARC (10-fold). Activation of DCs by CD40 Ligand further upregulated the expression of ELC (25-fold). We found that freshly isolated blood DCs expressed only low levels of interleukin-8, lymphotactin, and MIP-1 alpha. It is interesting that the chemokine profile expressed by activated CD11c(-) lymphoid-like as well as CD11c(+) myeloid blood DCs mimics that of monocyte-derived DCs, Additionally, purified Langerhans cells that had migrated out of the epidermis expressed a similar chemokine pattern, These data indicate that different DC subsets in vitro and in vivo can express the same chemokines to attract leukocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:48:03