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Titolo:
Biochemical and genetic bases of microbial degradation of polychlorinated biphenyls (PCBs)
Autore:
Furukawa, K;
Indirizzi:
Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Higashi Ku, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ci, Higashi Ku, Fukuoka 8128581, Japan
Titolo Testata:
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
fascicolo: 6, volume: 46, anno: 2000,
pagine: 283 - 296
SICI:
0022-1260(200012)46:6<283:BAGBOM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP STRAIN KKS102; PSEUDOMONAS-PSEUDOALCALIGENES KF707; RHODOCOCCUS-GLOBERULUS P6; 2,3-DIHYDROXYBIPHENYL DIOXYGENASE GENE; FUNGUS PHANEROCHAETE-CHRYSOSPORIUM; ALCALIGENES-EUTROPHUS H850; BPH OPERON; DEGRADING BACTERIUM; NUCLEOTIDE-SEQUENCE; ENCODING BIPHENYL;
Keywords:
biphenyl; biphenyl dioxygenase; bph genes; microbial degradation; molecular breeding; PCB; polychlorinated biphenyls;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
96
Recensione:
Indirizzi per estratti:
Indirizzo: Furukawa, K Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Higashi Ku, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 Ku, Fukuoka 8128581, Japan
Citazione:
K. Furukawa, "Biochemical and genetic bases of microbial degradation of polychlorinated biphenyls (PCBs)", J GEN A MIC, 46(6), 2000, pp. 283-296

Abstract

The microbial degradation of polychlorinated biphenyls (PCBs) has been extensively conducted by many workers, and the following general results have been obtained, (1) PCBs are degraded oxidatively by aerobic bacteria and other microorganisms such as white rot fungi. PCBs are also reductively dehalogenated by anaerobic microbial consortia, (2) The biodegradability of PCBsis highly dependent on chlorine substitution, i.e., number and position ofchlorine, The degradation and dehalogenation capabilities are also highly strain dependent, (3) Biphenyl-utilizing bacteria can cometabolize many PCBcongeners to chlorobenzoates by biphenl-catabolic enzymes. (4) Enzymes involved in the PCB degradation were purified and characterized, Biphenyl dioxygenase, ring-cleavage dioxygenase, and hydrolase are crystallized, and tworing-cleavage dioxygenases are being solved by x-ray crystallography. (5) The bph gene clusters responsible for PCB degradation are cloned from a variety of bacterial strains. The structure and function are analyzed with respect to the evolutionary relationship. (6) The molecular engineering of biphenyl dioxygenases is successfully performed by DNA shuffling, domain exchange, and subunit exchange. The evolved enzymes exhibit wide and enhanced degradation capacities for PCBs and other aromatic compounds.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 09:31:55