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Titolo:
PCR detection of 5 putative periodontal pathogens in dental plaque samplesfrom children 2 to 12 years of age
Autore:
Okada, M; Hayashi, F; Nagasaka, N;
Indirizzi:
Hiroshima Univ, Fac Dent, Dept Pediat Dent, Minami Ku, Hiroshima 7348553, Japan Hiroshima Univ Hiroshima Japan 7348553 nami Ku, Hiroshima 7348553, Japan
Titolo Testata:
JOURNAL OF CLINICAL PERIODONTOLOGY
fascicolo: 6, volume: 28, anno: 2001,
pagine: 576 - 582
SICI:
0303-6979(200106)28:6<576:PDO5PP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; BACTEROIDES-FORSYTHUS; PORPHYROMONAS-GINGIVALIS; SUBGINGIVAL PLAQUE; ADULT PERIODONTITIS; TREPONEMA-DENTICOLA; IDENTIFICATION; MICROORGANISMS; MICROBIOTA;
Keywords:
PCR; dental plaque; Prevotella intermedia; Prevotella nigrescens; Bacteroides forsythus; Treponema denticola; Campylobacter rectus; children;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Okada, M Hiroshima Univ, Fac Dent, Dept Pediat Dent, Minami Ku, 1-2-3 Kasumi, Hiroshima 7348553, Japan Hiroshima Univ 1-2-3 Kasumi Hiroshima Japan 7348553 48553, Japan
Citazione:
M. Okada et al., "PCR detection of 5 putative periodontal pathogens in dental plaque samplesfrom children 2 to 12 years of age", J CLIN PER, 28(6), 2001, pp. 576-582

Abstract

Background, aims: The purpose of this study was to detect the presence of Prevotella intermedia, P. nigrescens, Bacteroides forsythus, Treponema denticola, and Campylobacter rectus in plaque samples from 119 children, collected from their toothbrushes using a polymerase chain reaction (PCR). Method: The subjects were 24, 83, and 12 children with healthy gingiva, gingivitis, and periodontitis, respectively, ranging in age from 2-12 years old. Plaque samples were collected from all erupted teeth sites using a sterile toothbrush. The mean concentration of DNA recovered from the plaque samples was approximately 640 mug/ml, which was deemed sufficient for performing a PCR-based survey. Results: The prevalence by PCR in healthy, gingivitis, and periodontitis subjects was 0.0%, 6.0% and 25.0% for P. intermedia, 45.8%, 79.5% and 50.0% for P. nigrescens, 33.3%, 63.9% and 58.3% for B. forsythus, 0.0%, 18.1% and16.7% for T. denticola, and 100% in duplicate for C. rectus, respectively. Conclusion: Our survey indicated that P. intermedin and T. denticola were more associated with periodontal diseases, B. forsythus and P. nigrescens had a moderate prevalence in all clinical groups, while C. rectus were the most commonly detected species in the oral cavities of children suggesting establishment in their early years.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 10:15:19