Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Intramitochondrial location and dynamics of Crithidia fasciculata kinetoplast minicircle replication intermediates
Autore:
Drew, ME; Englund, PT;
Indirizzi:
Johns Hopkins Med Sch, Dept Biol Chem, Baltimore, MD 21205 USA Johns Hopkins Med Sch Baltimore MD USA 21205 hem, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 4, volume: 153, anno: 2001,
pagine: 735 - 743
SICI:
0021-9525(20010514)153:4<735:ILADOC>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL-DNA PRIMASE; CELL-CYCLE; TRYPANOSOMA-BRUCEI; NETWORKS; TOPOISOMERASE; LOCALIZATION; SEGREGATION; MECHANISM; SEQUENCE; NUCLEAR;
Keywords:
cell cycle; kinetoplast DNA; DNA replication; fluorescence in situ hybridization; trypanosomatid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Englund, PT Johns Hopkins Med Sch, Dept Biol Chem, 725 N Wolfe St, Baltimore, MD 21205USA Johns Hopkins Med Sch 725 N Wolfe St Baltimore MD USA 21205SA
Citazione:
M.E. Drew e P.T. Englund, "Intramitochondrial location and dynamics of Crithidia fasciculata kinetoplast minicircle replication intermediates", J CELL BIOL, 153(4), 2001, pp. 735-743

Abstract

Kinetoplast DNA, the mitochondrial DNA of Crithidia fasciculata, is organized into a network containing 5,000 topologically interlocked minicircles. This network, situated within the mitochondrial matrix, is condensed into adisk-shaped structure located near the basal body of the flagellum. Fluorescence in situ hybridization revealed that before their replication, minicircles are released vectorially from the network face nearest the flagellum. Replication initiates in the zone between the flagellar face of the disk and the mitochondrial membrane (we term this region the kinetoflagellar zone[KFZ]). The replicating minicircles then move to two antipodal sites that flank the disk-shaped network. In later stages of replication, the number of free minicircles increases, accumulating transiently in the KFZ. The final replication events, including primer removal, repair of many of the gaps,and reattachment of the progeny minicircles to the network periphery, are thought to take place within the antipodal sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:27:57