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Titolo:
Intramitochondrial localization of universal minicircle sequence-binding protein, a trypanosomatid protein that binds kinetoplast minicircle replication origins
Autore:
Abu-Elneel, K; Robinson, DR; Drew, ME; Englund, PT; Shlomai, J;
Indirizzi:
Hebrew Univ Jerusalem, Hadassah Med Sch, Kuvin Ctr Study Infect & Trop Dis, Dept Parasitol, IL-91120 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91120 -91120 Jerusalem, Israel Johns Hopkins Med Sch, Dept Biol Chem, Baltimore, MD 21205 USA Johns Hopkins Med Sch Baltimore MD USA 21205 hem, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 4, volume: 153, anno: 2001,
pagine: 725 - 733
SICI:
0021-9525(20010514)153:4<725:ILOUMS>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL-DNA PRIMASE; ZINC-FINGER PROTEIN; CRITHIDIA-FASCICULATA; CELL-CYCLE; TOPOISOMERASE-II; POLYMERASE-BETA; ORGANIZATION; EXPRESSION; SITES; IDENTIFICATION;
Keywords:
kinetoplast DNA; kDNA replication; universal minicircle sequence; UMS-binding protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Shlomai, J Hebrew Univ Jerusalem, Hadassah Med Sch, Kuvin Ctr Study Infect& Trop Dis, Dept Parasitol, IL-91120 Jerusalem, Israel Hebrew Univ Jerusalem Jerusalem Israel IL-91120 salem, Israel
Citazione:
K. Abu-Elneel et al., "Intramitochondrial localization of universal minicircle sequence-binding protein, a trypanosomatid protein that binds kinetoplast minicircle replication origins", J CELL BIOL, 153(4), 2001, pp. 725-733

Abstract

Kinetoplast DNA (kDNA), the mitochondrial DNA of the trypanosomatid Crithidia fasciculata, is a unique structure containing 5,000 DNA minicircles topologically linked into a massive network. In vivo, the network is condensedinto a disk-shaped structure. Replication of minicircles initiates at unique origins that are bound by universal minicircle sequence (UMS), binding protein (UMSBP), a sequence-specific DNA-binding protein. This protein, encoded by a nuclear gene, localizes within the cell's single mitochondrion. Using immunofluorescence, we found that UMSBP localizes exclusively to two neighboring sites adjacent to the face of the kDNA disk nearest the cell's flagellum. This site is distinct from the two antipodal positions at the perimeter of the disk that is occupied by DNA polymerase beta, topoisomerase II, and a structure-specific endonuclease. Although we found constant steady-state levels of UMSBP mRNA and protein and a constant rate of UMSBP synthesis throughout the cell cycle, immunofluorescence indicated that UMSBP localization within the kinetoplast is not static. The intramitochondrial localization of UMSBP and other kDNA replication enzymes significantly clarifies our understanding of the process of kDNA replication.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:16:31