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Titolo:
Simple purification methods for an alpha Galactose-specific antibody from chicken eggs
Autore:
Cook, CL; Pao, W; Firca, JR; Anderson, BE; Fryer, JP;
Indirizzi:
Northwestern Univ, Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 & Mol Biol, Chicago, IL 60611 USA Ophidian Pharmaceut Inc, Madison, WI 53711 USA Ophidian Pharmaceut Inc Madison WI USA 53711 t Inc, Madison, WI 53711 USA Northwestern Univ, Sch Med, Dept Surg, Chicago, IL 60611 USA Northwestern Univ Chicago IL USA 60611 , Dept Surg, Chicago, IL 60611 USA
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 3, volume: 91, anno: 2001,
pagine: 305 - 310
SICI:
1389-1723(200103)91:3<305:SPMFAA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERACTION CHROMATOGRAPHY; YOLK; IGY; IMMUNOGLOBULINS; EXTRACTION; LAID; HENS;
Keywords:
avian antibody; IgY; agalactose; thiophilic chromatography; affinity chromatography; lipid;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Anderson, BE Northwestern Univ, Sch Med, Dept Cell & Mol Biol, 303 E Chicago Ave, Chicago, IL 60611 USA Northwestern Univ 303 E Chicago Ave Chicago IL USA 60611 USA
Citazione:
C.L. Cook et al., "Simple purification methods for an alpha Galactose-specific antibody from chicken eggs", J BIOSCI BI, 91(3), 2001, pp. 305-310

Abstract

For many applications avian antibody from egg yolk (IgY) offers advantagesover the well-known mammalian antibodies. Different experimental techniques for the purification of IgY from chickens immunized with an agalactose-containing antigen (alpha Gal-trisaccharide) were compared. These included ammonium sulfate precipitation, filtration with diatomaceous earth, treatmentwith deoxycholate, and thiophilic and affinity chromatography. Samples were tested for overall purity, protein and lipid content, and specific activity. Evaluated on the basis of these results and the simplicity of the process, the favored purification method is ammonium sulfate precipitation of diluted egg yolk directly followed by affinity chromatography. The high Lipidcontent of IgY preparations is greatly reduced by either thiophilic or affinity chromatography. Affinity purification of ammonium sulfate precipitated material resulted in anti-alpha Gal-trisaccharide IgY preparations with approximately 1% of the original protein content but approximately 100-fold higher specific activity for the alpha Gal-trisaccharide epitope.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:42:41