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Titolo:
Involvement of formate as an interspecies electron carrier in a syntrophicacetate-oxidizing anaerobic microorganism in coculture with methanogens
Autore:
Hattori, S; Luo, HW; Shoun, H; Kamagata, Y;
Indirizzi:
Agcy Ind Sci & Technol, Natl Inst Biosci & Human Technol, Tsukuba, Ibaraki3058566, Japan Agcy Ind Sci & Technol Tsukuba Ibaraki Japan 3058566 baraki3058566, Japan Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058572, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058572 sukuba, Ibaraki 3058572, Japan
Titolo Testata:
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
fascicolo: 3, volume: 91, anno: 2001,
pagine: 294 - 298
SICI:
1389-1723(200103)91:3<294:IOFAAI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SP-NOV; BACTERIUM; DEGRADATION; OXIDATION; BUTYRATE; H-2; ASSOCIATION; PROPIONATE; METABOLISM; BRYANTII;
Keywords:
interspecies hydrogen transfer; interspecies formate transfer; syntrophic acetate oxidation; hydrogen partial pressures; Thermacetogenium phaeum; Methanothermobacter thermautotrophicus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kamagata, Y Agcy Ind Sci & Technol, Natl Inst Biosci & Human Technol, 1-1 Higashi, Tsukuba, Ibaraki 3058566, Japan Agcy Ind Sci & Technol 1-1 HigashiTsukuba Ibaraki Japan 3058566
Citazione:
S. Hattori et al., "Involvement of formate as an interspecies electron carrier in a syntrophicacetate-oxidizing anaerobic microorganism in coculture with methanogens", J BIOSCI BI, 91(3), 2001, pp. 294-298

Abstract

To determine whether formate is involved in interspecies electron transferbetween substrate-oxidizing bacteria and hydrogenotrophic microorganisms under anaerobic conditions, a syntrophic acetate-oxidizing bacterium Thermacetogenium phaeum strain PB was cocultured either with a formate/H-2-utilizing methanogen strain TM (designated as PB/TM coculture), or an H-2-utilizing methanogen strain DeltaH (designated as PB/DeltaH coculture). Acetate oxidation and subsequent methanogenesis in PB/TM coculture were found to be significantly faster than in PB/DeltaH coculture. Formate dehydrogenase and hydrogenase were both detected in strains PB and TM. H-2 partial pressures in the PB/TM coculture were kept tower (20 to 40 Pa) than those of the PB/DeltaH coculture (40 to 60 Pa) during the exponential growth phase. Formate was also detected in both PB/TM and PB/DeltaH cocultures, and the concentration of formate was maintained at a lower level in the PB/TM coculture (5 to9 muM) than in the PB/DeltaH coculture. Thermodynamic calculations revealed that the concentrations of both H-2 and formate severely affect the syntrophic oxidation of acetate. These results strongly indicate that not only H-2 but also formate may be involved in interspecies electron transfer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 05:23:15