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Titolo:
Routine experimental system for defining conditions used in photodynamic therapy and fluorescence photodetection of (non-) neoplastic epithelia
Autore:
Lange, N; Vaucher, L; Marti, A; Etter, AL; Gerber, P; van den Bergh, H; Jichlinski, P; Kucera, P;
Indirizzi:
Univ Lausanne, Inst Physiol, CH-1007 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1007 CH-1007 Lausanne, Switzerland CHUV Hosp, Dept Urol, CH-1015 Lausanne, Switzerland CHUV Hosp Lausanne Switzerland CH-1015 ol, CH-1015 Lausanne, Switzerland Univ Lausanne, Inst Organ Chem, CH-1015 Lausanne, Switzerland Univ Lausanne Lausanne Switzerland CH-1015 CH-1015 Lausanne, Switzerland Swiss Fed Inst Technol, Inst Environm Engn, DGR LPAS, CH-1015 Lausanne, Switzerland Swiss Fed Inst Technol Lausanne Switzerland CH-1015 ausanne, Switzerland
Titolo Testata:
JOURNAL OF BIOMEDICAL OPTICS
fascicolo: 2, volume: 6, anno: 2001,
pagine: 151 - 159
SICI:
1083-3668(200104)6:2<151:RESFDC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PROTOPORPHYRIN-IX; 5-AMINOLEVULINIC ACID-ESTERS; TOPICAL APPLICATION; IN-VITRO; CELL CARCINOMA; BLADDER-CANCER; CHOROIDAL NEOVASCULARIZATION; INTRAVESICAL INSTILLATION; CLINICAL-EXPERIENCE; TUMORS;
Keywords:
PDT; tissue culture; 5-aminolevulinic acid derivatives; protoporphyrin IX; fluorescence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Engineering, Computing & Technology
Citazioni:
52
Recensione:
Indirizzi per estratti:
Indirizzo: Lange, N Swiss Fed Inst Technol, Inst Environm Engn, DGR LPAS, CH-1015 Lausanne, Switzerland Swiss Fed Inst Technol Lausanne Switzerland CH-1015 Switzerland
Citazione:
N. Lange et al., "Routine experimental system for defining conditions used in photodynamic therapy and fluorescence photodetection of (non-) neoplastic epithelia", J BIOMED OP, 6(2), 2001, pp. 151-159

Abstract

A common method to induce enhanced short-term endogenous porphyrin synthesis and accumulation in cell is the topical, systemic application of 5-aminolevulinic acid or one of its derivatives. This circumvents the intravenous administration of photosensitizers normally used for photodynamic therapy (PDT) of fluorescence photodetection. However, in the majority of potential medical indications, optimal conditions with respect to the porphyrin precursor or its pharmaceutical formulation have not yet been found. Due to ethical restrictions and animal right directives, the number of available test objects is limited. Hence, definition and use of nonanimal test methods areneeded. Tissue and organ cultures are a promising approach in replacing cost intensive animal models in early stages of drug development. In this paper, we present a tissue culture, which can among others be used routinely to answer specific questions emerging in the field of photodynamic therapy and fluorescence photodetection. This technique uses mucosae excised from sheep paranasal sinuses or pig bladder, which is cultured under controlled conditions. It allows quasiquantitative testing of different protoporphyrin IX precursors with respect to dose-response curves and pharmacokinetics, as well as the evaluation of different incubation conditions and/or different drug formulations. Furthermore, this approach, when combined with the use of electron microscopy and fluorescence-based methods, can be used to quantitatively determine the therapeutic outcome following protoporphyrin IX-mediated PDT. (C) 2001 Society or Photo-Optical Instrumentation Engineers.

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Documento generato il 06/04/20 alle ore 08:36:25