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Titolo:
Indirect role for COPI in the completion of Fc gamma receptor-mediated phagocytosis
Autore:
Hackam, DJ; Botelho, RJ; Sjolin, C; Rotstein, OD; Robinson, JM; Schreiber, AD; Grinstein, S;
Indirizzi:
Hosp Sick Children, Div Cell Biol, Toronto, ON M5G 1X8, Canada Hosp Sick Children Toronto ON Canada M5G 1X8 Toronto, ON M5G 1X8, Canada Toronto Gen Hosp, Dept Surg, Toronto, ON M5G 2C4, Canada Toronto Gen HospToronto ON Canada M5G 2C4 g, Toronto, ON M5G 2C4, Canada Ohio State Univ, Dept Physiol, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 ept Physiol, Columbus, OH 43210 USA Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PAUSA 19104 Dept Med, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 21, volume: 276, anno: 2001,
pagine: 18200 - 18208
SICI:
0021-9258(20010525)276:21<18200:IRFCIT>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADP-RIBOSYLATION FACTOR; SYK TYROSINE KINASE; PLASMA-MEMBRANE; EPSILON-COP; TRANSFERRIN RECEPTOR; GOLGI MEMBRANES; CELL MUTANT; BREFELDIN-A; CHO CELLS; MACROPHAGES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Grinstein, S Hosp Sick Children, Div Cell Biol, 555 Univ Ave, Toronto, ON M5G 1X8, Canada Hosp Sick Children 555 Univ Ave Toronto ON Canada M5G 1X8 ada
Citazione:
D.J. Hackam et al., "Indirect role for COPI in the completion of Fc gamma receptor-mediated phagocytosis", J BIOL CHEM, 276(21), 2001, pp. 18200-18208

Abstract

Recent evidence suggests that extension of pseudopods during phagocytosis requires localized insertion of endomembrane vesicles. The nature of these vesicles and the processes mediating their release and insertion are unknown. COPI plays an essential role in the budding and traffic of membrane vesicles in intracellular compartments. We therefore assessed whether COPI is also involved in phagosome formation. We used IdlF cells, a mutant line derived from Chinese hamster ovary cells that express a temperature-sensitive form of epsilon COP, To confer phagocytic ability to IdlF cells, they were stably transfected with Fc receptors type IIA (Fc gamma RIIA), In the presence of functional COPI, Fc gamma RIIA-transfected IdlF cells effectively internalized opsonized particles. In contrast, phagocytosis was virtually eliminated after incubation at the restrictive temperature. Similar results were obtained impairing COPI function in macrophages using brefeldin A. Notably, loss of COPI function preceded complete inhibition of phagocytosis, suggesting that COPI is indirectly required for phagocytosis, Despite their inability to internalize particles, COPI-deficient cells nevertheless expressed normal levels of Fc gamma RIIA, and signal transduction appeared unimpeded, The opsonized particles adhered normally to COPI-deficient cells and were often found on actin-rich pedestals, but they were not internalized due to the inability of the cells to extend pseudopods. The failure to extend pseudopods was attributed to the inability of COPI-deficient cells to mobilize endomembrane vesicles, including a VAMP3-containing compartment, in response to the phagocytic stimulus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:08:15