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Titolo:
Sequential involvement of P115, SNAREs, and Rab proteins in intra-Golgi protein transport
Autore:
Gmachl, MJS; Wimmer, C;
Indirizzi:
Basel Inst Immunol, CH-4005 Basel, Switzerland Basel Inst Immunol Basel Switzerland CH-4005 CH-4005 Basel, Switzerland Inst Mol Pathol, A-1030 Vienna, Austria Inst Mol Pathol Vienna Austria A-1030 Mol Pathol, A-1030 Vienna, Austria
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 21, volume: 276, anno: 2001,
pagine: 18178 - 18184
SICI:
0021-9258(20010525)276:21<18178:SIOPSA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GTP-BINDING PROTEIN; SENSITIVE FUSION PROTEIN; DISSOCIATION INHIBITOR ISOFORM; HOMOTYPIC VACUOLE FUSION; CELL-FREE SYSTEM; MEMBRANE-FUSION; VESICULAR TRANSPORT; ALPHA-SNAP; MOLECULAR-CLONING; SYNTAXIN FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
87
Recensione:
Indirizzi per estratti:
Indirizzo: Wimmer, C Basel Inst Immunol, Grenzacherstr 487, CH-4005 Basel, Switzerland Basel Inst Immunol Grenzacherstr 487 Basel Switzerland CH-4005
Citazione:
M.J.S. Gmachl e C. Wimmer, "Sequential involvement of P115, SNAREs, and Rab proteins in intra-Golgi protein transport", J BIOL CHEM, 276(21), 2001, pp. 18178-18184

Abstract

Delivery of transport vesicles to their receptor compartment involves tethering, priming, and fusion. Soluble NSF attachment protein-alpha (alpha SNAP) mediates the disruption of SNAREs by N-ethylmaleimide sensitive factor (NSF) and was employed to determine the hierarchy of proteins responsible for intra-Golgi protein transport. The N-terminal 23 amino acids of alpha SNAP are necessary for SNARE binding. The antibody 2F10 recognizes this SNARE interaction domain of alpha SNAP and inhibits intra-Golgi protein transportreversibly. This antibody was applied to modify the transport assay to determine the protein requirements relative to the action of alpha SNAP and NSF. We found that 1) p115 acts independently of alpha SNAP and NSF, 2) SNAREs are required after tethering and interact selectively after activation byalpha SNAP and NSF, and 3) Rab proteins act after SNARE activation and before fusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:04:57