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Titolo:
Dexamethasone increases the expression of membrane macrophage colony stimulating factor from retrovirally transduced tumor cells expressing macrophage colony stimulating factor
Autore:
Dan, Q; Trinh, H; Williams, CC; Lloyd, C; Wepsic, HT; Jeffes, EWB; Jadus, MR;
Indirizzi:
Vet Affairs Med Ctr, Dept Lab Serv, Diagnost & Mol Med Hlth Care Grp, LongBeach, CA 90822 USA Vet Affairs Med Ctr Long Beach CA USA 90822 Grp, LongBeach, CA 90822 USA Univ Calif Irvine, Dept Pathol, Irvine, CA 92117 USA Univ Calif Irvine Irvine CA USA 92117 , Dept Pathol, Irvine, CA 92117 USA Vet Affairs Med Ctr, Dept Dermatol, Long Beach, CA 90822 USA Vet Affairs Med Ctr Long Beach CA USA 90822 tol, Long Beach, CA 90822 USA Univ Calif Irvine, Dept Dermatol, Irvine, CA 92117 USA Univ Calif Irvine Irvine CA USA 92117 Dept Dermatol, Irvine, CA 92117 USA
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 4, volume: 1, anno: 2001,
pagine: 737 - 748
SICI:
1567-5769(200104)1:4<737:DITEOM>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FMS CSF-1 RECEPTOR; MAMMARY ADENOCARCINOMA; ECTODOMAIN CLEAVAGE; BREAST CARCINOMAS; IMMUNITY; LYMPHOCYTES; INHIBITION; REJECTION; ISOFORM; CANCER;
Keywords:
macrophage colony-stimulating factor; tumor cells; dexamethasone; macrophage-mediated cytotoxicity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Jadus, MR Vet Affairs Med Ctr, Dept Lab Serv, Diagnost & Mol Med Hlth CareGrp, 5901E 7th St,Box 113, Long Beach, CA 90822 USA Vet Affairs Med Ctr 5901 E 7th St,Box 113 Long Beach CA USA 90822
Citazione:
Q. Dan et al., "Dexamethasone increases the expression of membrane macrophage colony stimulating factor from retrovirally transduced tumor cells expressing macrophage colony stimulating factor", INT IMMUNO, 1(4), 2001, pp. 737-748

Abstract

Many different tumor cell types (breast, ovarian, glioma, liver and colon)were retrovirally transduced with the human macrophage colony stimulating factor (M-CSF) gene (either the membrane associated form [mM-CSF] or the secreted form [sM-CSF]). These cells were tested for their ability to displayincreased amounts of mM-CSF in response to dexamethasone. M-CSF-transfected tumor cells expressed additional mM-CSF in response to 18-72 h incubations with 3-15 mug/ml dexamethasone, while non-transfected parental cells wereunaffected by this treatment, increased mM-CSF protein expression on the M-CSF transduced cells was observed by flow cytometry and Western blotting using M-CSF specific antibodies. Northern blot analysis revealed an increasein the mM-CSF specific transcripts within the dexamethasone-treated mM-CSFtransduced cells, but this was not seen within the non-transfected tumor cells that were treated with dexamethasone. ICAM-1 expression was unaffectedby dexamethasone treatment, indicating that this response is mM-CSF specific. All trans-retinal and 1,25-dihydroxy vitamin D3 compounds that have been reported to induce M-CSF expression failed to increase mM-CSF. When dexamethasone-treated mM-CSF transfected clones were used as target cells for macrophage mediated cytotoxicity assays, an increased killing with the dexamethasone-treated cells was seen. The macrophage-mediated cytotoxicity of these mM-CSF expressing tumor cells was blocked with excess recombinant M-CSF by saturating M-CSF receptors on the macrophage that is required for this form of tumor cell killing. This work suggests the possibility that dexamethasone may prove useful for vaccination purposes using mM-CSF retrovirally transfected tumor cells. Published by Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 18:46:01