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Titolo:
Recombinant expression of human mannan-binding lectin
Autore:
Vorup-Jensen, T; Sorensen, ES; Jensen, UB; Schwaeble, W; Kawasaki, T; Ma, Y; Uemura, K; Wakamiya, N; Suzuki, Y; Jensen, TG; Takahashi, K; Ezekowitz, RAB; Thiel, S; Jensenius, JC;
Indirizzi:
Univ Aarhus, Dept Med Microbiol & Immunol, DK-8000 Aarhus C, Denmark Univ Aarhus Aarhus Denmark C robiol & Immunol, DK-8000 Aarhus C, Denmark Aarhus Univ, Prot Lab, DK-8000 Aarhus, Denmark Aarhus Univ Aarhus Denmark DK-8000 iv, Prot Lab, DK-8000 Aarhus, Denmark Aarhus Univ, Dept Human Genet, DK-8000 Aarhus C, Denmark Aarhus Univ Aarhus Denmark C Dept Human Genet, DK-8000 Aarhus C, Denmark Univ Leicester, Dept Microbiol & Immunol, Leicester LE1 9HN, Leics, England Univ Leicester Leicester Leics England LE1 9HN er LE1 9HN, Leics, England Kyoto Univ, Grad Sch Pharmaceut Sci, Japan Sci & Technol Corp, Dept Biol Chem,Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 Biol Chem,Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ, Grad Sch Pharmaceut Sci, Japan Sci & Technol Corp, Core Res Evolut Sci & Technol Project,Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 ol Project,Sakyo Ku, Kyoto 6068501, Japan Osaka Univ, Osaka Prefectural Inst Publ Hlth, Microbial Dis Res Inst, Suita, Osaka, Japan Osaka Univ Suita Osaka Japan Microbial Dis Res Inst, Suita, Osaka, Japan Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Pediat,Lab Dev Immunol, Boston, MA 02114 USA Harvard Univ Boston MA USA 02114 at,Lab Dev Immunol, Boston, MA 02114 USA
Titolo Testata:
INTERNATIONAL IMMUNOPHARMACOLOGY
fascicolo: 4, volume: 1, anno: 2001,
pagine: 677 - 687
SICI:
1567-5769(200104)1:4<677:REOHML>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC-LUPUS-ERYTHEMATOSUS; COLLAGEN-LIKE DOMAIN; RHEUMATOID-ARTHRITIS; SERINE-PROTEASE; CYSTIC-FIBROSIS; STRUCTURAL GENE; MANNOSE; COMPLEMENT; SERUM; ASSOCIATION;
Keywords:
structural and functional comparison recombinant mannan-binding lectins carbohydrate affinity chromatography complement deficiency;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Vorup-Jensen, T Univ Aarhus, Dept Med Microbiol & Immunol, Bartholin Bldg,Wilhelm Meyers Alle, DK-8000 Aarhus C, Denmark Univ Aarhus Bartholin Bldg,Wilhelm Meyers Alle Aarhus Denmark C
Citazione:
T. Vorup-Jensen et al., "Recombinant expression of human mannan-binding lectin", INT IMMUNO, 1(4), 2001, pp. 677-687

Abstract

Mannan-binding lectin (MBL) constitutes an important part of the innate immune defend by effecting the deposition of complement on microbial surfaces. MBL deficiency is among the most common primary immunodeficiencies and isassociated with recurrent infections and symptoms of poor immune complex clearance. Plasma-derived MBL has been used in reconstitution therapy but concerns over viral contamination and production capacity point to recombinant MBL (rMBL) as a future source of this protein for clinical use. Natural human MBL is an oligomer of up to 18 identical polypeptide chains. The synthesis of rMBL has been accomplished in several mammalian cell lines, however, the recombinant protein differed structurally from natural MEL. In this, study we compare rMBL produced in myeloma cells, Chinese hamster ovary (CHO) cells, human hepatocytes, and human embryonic kidney (HEK) cells. We report that rMBL structurally and functionally similar to natural MBL can be obtained through synthesis in the human embryonic kidney cells followed by selective carbohydrate affinity chromatography. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/12/19 alle ore 04:07:33