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Titolo:
Regulation of surface Fc epsilon RI expression an human eosinophils by IL-4 and IgE
Autore:
Iikura, M; Yamaguchi, M; Hirai, K; Miyamasu, M; Yamada, H; Nakajima, T; Fujisawa, T; Ra, C; Morita, Y; Yamamoto, K;
Indirizzi:
Univ Tokyo, Grad Sch Med, Dept Allergy & Rheumatol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 heumatol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo, Grad Sch Med, Dept Bioregulatory Funct, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 ry Funct, Bunkyo Ku, Tokyo 1138655, Japan Natl Mie Hosp, Dept Pediat, Tsu, Mie, Japan Natl Mie Hosp Tsu Mie JapanNatl Mie Hosp, Dept Pediat, Tsu, Mie, Japan Juntendo Univ, Sch Med, Allergy & Atopy Res Ctr, Tokyo 113, Japan JuntendoUniv Tokyo Japan 113 Allergy & Atopy Res Ctr, Tokyo 113, Japan Univ Tokyo, Grad Sch Med, Dept Resp Med, Tokyo, Japan Univ Tokyo Tokyo Japan Tokyo, Grad Sch Med, Dept Resp Med, Tokyo, Japan
Titolo Testata:
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
fascicolo: 4, volume: 124, anno: 2001,
pagine: 470 - 477
SICI:
1018-2438(200104)124:4<470:ROSFER>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH-AFFINITY RECEPTOR; EPIDERMAL LANGERHANS CELLS; CHAIN MESSENGER-RNA; MAST-CELLS; INTRACELLULAR EXPRESSION; HUMAN PLATELETS; I EXPRESSION; B-CELLS; BASOPHILS; ALPHA;
Keywords:
eosinophils; Fc epsilon RI; IgE; IL-4; flow cytometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, M Univ Tokyo, Grad Sch Med, Dept Allergy & Rheumatol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655, Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 yo 1138655, Japan
Citazione:
M. Iikura et al., "Regulation of surface Fc epsilon RI expression an human eosinophils by IL-4 and IgE", INT A AL IM, 124(4), 2001, pp. 470-477

Abstract

Background: Recent studies have demonstrated that eosinophils from allergic patients express low levels of Fc epsilon RI on their surface, but the regulatory mechanisms of eosinophil surface Fc epsilon RI expression are not fully understood. We investigated whether IL-4 and IgE, which are reported to regulate surface Fc epsilon RI expression on human mast cells, are able to affect surface Fc epsilon RI expression in normal human eosinophils. Methods: Eosinophils purified from peripheral blood were cultured with IL-5 and with or without IL-4 and/or IgE, and surface Fc epsilon RI expression wasanalyzed by flow cytometry using an anti-Fc epsilon RI mAb, CRA-1. Results: Apparent Fc epsilon RI expression (similar to 1% of mast cell Fc epsilon RI levels) was observed in eosinophils cultured with both IL-4 and IgE. A combination of IL-4 (greater than or equal to 1 ng/ml) and IgE (greater thanor equal to 0.5 mug/ml) was necessary for the maximal induction of surfaceFc epsilon RI expression. In the presence of IL-4 and IgE, eosinophils cultured for 2 days demonstrated low but statistically significant levels of surface Fc epsilon RI, which reached a plateau after 7 days of culture. However, cross-linkage of surface Fc epsilon RI molecules by CRA-1 or anti-IgE did not induce any eosinophil activation. Conclusions: IL-4 and IgE can affect the levels of surface Fc epsilon RI on normal human eosinophils. Fc epsilon RI expression on eosinophils may be regulated by a mechanism similar to that in mast cells. Copyright (C) 2001 S. Karger AG, Basel.

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Documento generato il 26/01/20 alle ore 09:48:52