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Titolo:
Selection with melphalan or paclitaxel (Taxol) yields variants with different patterns of multidrug resistance, integrin expression and in vitro invasiveness
Autore:
Liang, Y; Meleady, P; Cleary, I; McDonnell, S; Connolly, L; Clynes, M;
Indirizzi:
Dublin City Univ, Natl Inst Cellular Biotechnol, Natl Cell & Tissue Culture Ctr, Dublin 9, Ireland Dublin City Univ Dublin Ireland 9 Tissue Culture Ctr, Dublin 9, Ireland Dublin City Univ, Sch Biotechnol, Dublin 9, Ireland Dublin City Univ Dublin Ireland 9 niv, Sch Biotechnol, Dublin 9, Ireland
Titolo Testata:
EUROPEAN JOURNAL OF CANCER
fascicolo: 8, volume: 37, anno: 2001,
pagine: 1041 - 1052
SICI:
0959-8049(200105)37:8<1041:SWMOP(>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
CARCINOMA CELL-LINE; BREAST-CANCER CELLS; DRUG-RESISTANCE; TUMOR-CELLS; MURINE TUMOR; PROTEIN GENE; ALPHA-4-BETA-1; FIBRONECTIN; GELATINASE; METASTASIS;
Keywords:
melphalan; paclitaxel (taxol); multidrug resistance (MDR); invasion; multidrug resistance protein (MRP); integrin; matrix metalloproteinase (MMP);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Clynes, M Dublin City Univ, Natl Inst Cellular Biotechnol, Natl Cell & Tissue Culture Ctr, Dublin 9, Ireland Dublin City Univ Dublin Ireland 9 lture Ctr, Dublin 9, Ireland
Citazione:
Y. Liang et al., "Selection with melphalan or paclitaxel (Taxol) yields variants with different patterns of multidrug resistance, integrin expression and in vitro invasiveness", EUR J CANC, 37(8), 2001, pp. 1041-1052

Abstract

A melphalan-resistant variant (Roswell Park Memorial Institute (RPMI)-2650M1) and a paclitaxel-resistant variant (RPMI-1650Tx) of the drug-sensitive human nasal carcinoma cell line, RPMI-2650. were established. The multidrugresistance (MDR) phenotype in the RPMI-2650Tx appeared to be P-glycoprotein (PgP)-mediated. Overexpression of multidrug resistant protein (MRP) family members was observed in the RPMI-2650M1 cells, which were also much more invasive in vitro than the parental cell line or the paclitaxel-resistant variant. Increased expression of alpha (2), alpha (5), alpha (6), beta (1) and beta (4) integrin subunits, decreased expression of alpha (4) integrin subunit, stronger adhesion to collagen type IV, laminin, fibronectin and matrigel, increased expression of MMP-2 and MMP-9 and significant motility compared with the parental cells were observed, along with a high invasivenessin the RPMI-7650M1 cells. Decreased expression of the alpha (2) integrin subunit, decreased attachment to collagen type IV, absence of cytokeratin 18expression, no detectable expression of gelatin-degrading proteases and poor motility may be associated with the non-invasiveness of the RPMI-2650Tx variant. These results suggest that melphalan exposure can result in not only a MDR phenotype. but could also make cancer cells more invasive, whereaspaclitaxel exposure resulted in MDR without increasing the in vitro invasiveness in the RPMI-2650 cells. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 25/09/20 alle ore 22:52:49