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Titolo:
Characterization of (R/S)-mecoprop [2-(2-methyl-4-chlorophenoxy) propionicacid]-degrading Alcaligenes sp CS1 and Ralstonia sp CS2 isolated from agricultural soils
Autore:
Smejkal, CW; Vallaeys, T; Seymour, FA; Burton, SK; Lappin-Scott, HM;
Indirizzi:
Univ Exeter, Sch Biol Sci, Exeter EX4 4PS, Devon, England Univ Exeter Exeter Devon England EX4 4PS , Exeter EX4 4PS, Devon, England CEN SCK, Microbiol Lab, Radioact Waste & Clean Up Div, B-2400 Mol, BelgiumCEN SCK Mol Belgium B-2400 act Waste & Clean Up Div, B-2400 Mol, Belgium
Titolo Testata:
ENVIRONMENTAL MICROBIOLOGY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 288 - 293
SICI:
1462-2912(200104)3:4<288:CO([P>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
2,4-DICHLOROPHENOXYACETIC ACID DEGRADATION; 2,4,5-TRICHLOROPHENOXYACETIC ACID; EUTROPHUS JMP134; ENANTIOSELECTIVE DEGRADATION; BACTERIAL CULTURES; GENE-TRANSFER; PLASMID PJP4; MECOPROP; BIODEGRADATION; EVOLUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Smejkal, CW Univ Exeter, Sch Biol Sci, Prince Wales Rd, Exeter EX4 4PS, Devon, England Univ Exeter Prince Wales Rd Exeter Devon England EX4 4PS gland
Citazione:
C.W. Smejkal et al., "Characterization of (R/S)-mecoprop [2-(2-methyl-4-chlorophenoxy) propionicacid]-degrading Alcaligenes sp CS1 and Ralstonia sp CS2 isolated from agricultural soils", ENVIRON MIC, 3(4), 2001, pp. 288-293

Abstract

The herbicide mecoprop [2-(2-methyl-4-chlorophenoxy) propionic acid] is widely applied to corn fields in order to control broad-leaved weeds. However, it is often detected in groundwater where it can be a persistent contaminant, Two mecoprop-degrading bacterial strains were isolated from agricultural soils through their capability to degrade (R/S)-mecoprop rapidly, 16S rDNA sequencing of the isolates demonstrated that one was closely related to the genera Alcaligenes sp. (designated CS1) and the other to Ralstonia sp. (designated CS2), Additionally, these isolates demonstrated ability to growon other related herbicides, including 2,4-D (2,4-dichlorophenoxyacetic acid), MCPA [4-chloro-2-methyl phenoxy acetic acid] and (R/S)-2,4-DP [2-(2,4-dichlorophenoxy)propionic acid] as sole carbon sources, tfdABC gene-specific probes derived from the 2,4-D-degrading Variovorax paradoxus TV1 were used in hybridization analyses to establish whether tfd-like genes are presentin mecoprop-degrading bacteria. Hybridization analysis demonstrated that both Alcaligenes sp. CS1 and Ralstonia sp. CS2 harboured tfdA, tfdB and tfdCgenes on plasmids that have approximately > 60% sequence similarity to thetfdA, tfdB and tfdC genes of V. paradoxus, It is therefore likely that tfd-like genes may be involved in the degradation of mecoprop, and we are currently investigating this further.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 12:44:21