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Titolo:
Ribotyping of rhizobia nodulating Acacia mangium and Paraserianthes falcataria from different geographical areas in Indonesia using PCR-RFLP-SSCP (PRS) and sequencing
Autore:
Clapp, JP; Mansur, I; Dodd, JC; Jeffries, P;
Indirizzi:
Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England Univ Kent Canterbury Kent England CT2 7NJ nterbury CT2 7NJ, Kent, England Int Inst Biotechnol, Sittingbourne Res Ctr, Sittingbourne ME9 8HL, Kent, England Int Inst Biotechnol Sittingbourne Kent England ME9 8HL 8HL, Kent, England
Titolo Testata:
ENVIRONMENTAL MICROBIOLOGY
fascicolo: 4, volume: 3, anno: 2001,
pagine: 273 - 280
SICI:
1462-2912(200104)3:4<273:RORNAM>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND-CONFORMATION POLYMORPHISM; RIBOSOMAL-RNA GENES; POLYMERASE-CHAIN-REACTION; ROOT-NODULE BACTERIA; BRADYRHIZOBIUM STRAINS; SP-NOV; DIVERSITY; IDENTIFICATION; JAPONICUM; PHYLOGENY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Jeffries, P Univ Kent, Res Sch Biosci, Canterbury CT2 7NJ, Kent, England Univ Kent Canterbury Kent England CT2 7NJ 7NJ, Kent, England
Citazione:
J.P. Clapp et al., "Ribotyping of rhizobia nodulating Acacia mangium and Paraserianthes falcataria from different geographical areas in Indonesia using PCR-RFLP-SSCP (PRS) and sequencing", ENVIRON MIC, 3(4), 2001, pp. 273-280

Abstract

Acacia mangium and Paraserianthes falcataria are leguminous tree species widely grown for timber in Indonesia and other tropical countries, yet little is known about the identity of their rhizobial symbionts, Polymerase chain reaction-restriction fragment length polymorphism-single-strand conformational polymorphism (PRS) analysis of the 16S rRNA gene was used along with sequencing to assess the diversity of 57 rhizobia isolated from nodules of A. mangium and P. falctaria in indonesia, In total, 26 rhizobia isolated from A. mangium were analysed by PRS and sequencing. The PRS patterns indicated that 12 (46%) clustered with Bradyrhizobium elkanii, 13 (50%) with B, lianoningense/japonicum and one (4%) with Mesorhizobium loti, Thirty-one isolates were analysed from P, falcataria: five (16%) clustered with B, elkaniiand 26 (84%) with S, lianoningense/ japonicum. These results were confirmed by phylogenetic analysis of sequences. Intraspecific diversity of the 16SrRNA genes from rhizobia nodulating A. mangium and P, falcataria revealed by PRS was low, only one genotype was found within the isolates that clustered with B. elkanii and two within the S, liaoningense/japonicum group. These Bradyrhizobium species are apparently ubiquitous throughout the Indonesian archipelago and it is clear why the two tree species are able to successfully establish outside their native range without the need for inoculationwith indigenous rhizobia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 23:36:00