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Titolo:
Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture
Autore:
Sasayama, N; Kashiwakura, I; Tokushima, Y; Xu, Y; Wada, S; Murakami, M; Hayase, Y; Takagi, Y; Takahashi, TA;
Indirizzi:
Univ Tokyo, Inst Med Sci, Cell Proc Dept, Minato Ku, Tokyo 1080071, Japan Univ Tokyo Tokyo Japan 1080071 roc Dept, Minato Ku, Tokyo 1080071, Japan Hokkaido Coll Pharm, Lab Radiopharmaceut Sci, Otaru, Hokkaido, Japan Hokkaido Coll Pharm Otaru Hokkaido Japan eut Sci, Otaru, Hokkaido, Japan
Titolo Testata:
CYTOTHERAPY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 117 - 126
SICI:
1465-3249(2001)3:2<117:EOMPFC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
EX-VIVO EXPANSION; HEMATOPOIETIC STEM-CELLS; COLONY-STIMULATING FACTOR; HIGH-DOSE CHEMOTHERAPY; IN-VITRO EXPANSION; BONE-MARROW; CD34(+) CELLS; SELF-RENEWAL; TRANSPLANTATION; RECONSTITUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sasayama, N Univ Tokyo, Inst Med Sci, Cell Proc Dept, Minato Ku, 4-6-1 Shirokanedai, Tokyo 1080071, Japan Univ Tokyo 4-6-1 Shirokanedai Tokyo Japan 1080071 0071, Japan
Citazione:
N. Sasayama et al., "Expansion of megakaryocyte progenitors from cryopreserved leukocyte concentrates of human placental and umbilical cord blood in short-term liquid culture", CYTOTHERAPY, 3(2), 2001, pp. 117-126

Abstract

Long-term severe thrombocytopenia following human placental and umbilical cord blood (CB) transplantation is a significant clinical problem. We studied the ex vivo expansion of megakaryocytic progenitor cells (CFU-Meg) from cryopreserved/thawed leukocyte concentrates (LC) of CB prepared by the Tokyo Cord Blood Bank protocol. The LC cells were cultured in serum-free culture medium supplemented with a combination of early-acting cytokines including thrombopoietin (TPO), flt3-ligand (FL), and stem cell factor (SCF). Combination of TPO plus FL, TPO plus SCF, and all of these cytokines together resulted in 8.9-, 7.7-, and 8.4-fold increases in CFU-Meg, respectively, by Day 5 of culture. Our results showed that this simple expansion strategy hasthe potential for expanding CFU-Meg from cryopreserved/thawed LC cells from CB.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 21:30:28