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Titolo:
Analytical performance of a direct assay for LDL-cholesterol
Autore:
Smets, EML; Pequeriaux, NCV; Blaton, V; Goldschmidt, HMJ;
Indirizzi:
St Elisabeth Zienkenhuis, Cent Klin Chem Hematol Lab, NL-5022 GC Tilburg, Netherlands St Elisabeth Zienkenhuis Tilburg Netherlands NL-5022 GC urg, Netherlands AZ St Jan Brugge, Lab Scheikunde, Brugge, Belgium AZ St Jan Brugge Brugge Belgium Brugge, Lab Scheikunde, Brugge, Belgium
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 3, volume: 39, anno: 2001,
pagine: 270 - 280
SICI:
1434-6621(200103)39:3<270:APOADA>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENSITY-LIPOPROTEIN CHOLESTEROL; CLINICAL-CHEMISTRY; SERUM;
Keywords:
lipids; LDL-cholesterol; direct assay; analytical performance;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Smets, EML St Elisabeth Zienkenhuis, Cent Klin Chem Hematol Lab, Hilvarenbeekseweg 60, NL-5022 GC Tilburg, Netherlands St Elisabeth Zienkenhuis Hilvarenbeekseweg 60 Tilburg Netherlands NL-5022 GC
Citazione:
E.M.L. Smets et al., "Analytical performance of a direct assay for LDL-cholesterol", CLIN CH L M, 39(3), 2001, pp. 270-280

Abstract

evaluated a direct assay for the determination of LDL-cholesterol (LDL-C) L-Type assay, Wake Pure Chemicals in two laboratories. This assay is applicable to most random access clinical chemistry analyzers, allowing full automation. Between-run coefficient of variation (NCCLS EP5) varied between 1.29% and 3.13% and thus met the National Cholesterol Education Program (NCEP) goal. The assay was considered linear over a physiologically relevant range of LDL-C, 2.22 to 7.04 mmol/l (NCCLS EP6). Method comparison yielded identical results at both evaluation sites for LDL-C when assayed with the direct method. LDL-C results obtained with the homogeneous method under investigation (y) differed significantly from values from density-gradient ultracentrifugation (x) according to Chung (y = 0.87x + 0.43 mmol/l, s(yx) = 0.38 mmol/l, r = 0.91). With the latter method asa reference method, mean bias was 3.16% meeting the NCEP criteria. Diagnostic performance was excellent at a clinically relevant cut-off level of 3.37 mmol/l. Results of the direct method (y) and the commonly used Friedewaldformula (x) were highly correlated (s(yx) = 0.22 mmol/l, r = 0.97), but both slope and intercept differed significantly from one and zero respectively (y = 0.90x + 0.37 mmol/l). Bilirubin, hemolysis and ascorbate did not interfere; triglycerides did not cause clinically relevant interference below 11.3 mmol/l. The direct method we investigated is user-friendly and provides an improvement in the determination of LDL-C in routine laboratories.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 03:46:38