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Titolo:
Calibration, specificity and trueness of a postheparin plasma lipoprotein lipase assay
Autore:
Henriksen, FL; Petersen, PH; Beck-Nielsen, H; Horder, M;
Indirizzi:
Univ So Denmark, Dept Clin Biochem & Clin Genet, Odense, Denmark Univ So Denmark Odense Denmark in Biochem & Clin Genet, Odense, Denmark Univ So Denmark, Dept Endocrinol, Odense, Denmark Univ So Denmark OdenseDenmark enmark, Dept Endocrinol, Odense, Denmark
Titolo Testata:
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
fascicolo: 3, volume: 39, anno: 2001,
pagine: 263 - 269
SICI:
1434-6621(200103)39:3<263:CSATOA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
FAMILIAL COMBINED HYPERLIPIDEMIA; FREQUENTLY OCCURRING MUTATION; CORONARY-ARTERY DISEASE; HEPATIC LIPASE; SELECTIVE MEASUREMENT; HDL CHOLESTEROL; GENE; TRIGLYCERIDE; METABOLISM; ASN291SER;
Keywords:
lipoprotein lipase activity; calibration system; analytical within-run variation; analytical between-run variation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Henriksen, FL Odense Univ Hosp, Dept Clin Biochem & Clin Genet, Sdr Blvd 29, D-5000 Cologne C, Germany Odense Univ Hosp Sdr Blvd 29 Cologne Germany C e C, Germany
Citazione:
F.L. Henriksen et al., "Calibration, specificity and trueness of a postheparin plasma lipoprotein lipase assay", CLIN CH L M, 39(3), 2001, pp. 263-269

Abstract

Measurement of lipoprotein lipase activity in postheparin plasma is generally accompanied by moderate within-run variation CVW-R (< 10%) and higher between-run variation CVB-R (5-25%). A calibration system was introduced in order to improve the reproducibility of measurements and to compare lipoprotein lipase activities from different days. Every day a calibration curve for lipoprotein lipase activity was constructed. Fifteen calibration curves designed over 2 years, show linearity over the whole biological spectrum and a considerable reduction of between-run variation in lipoprotein lipase activity, from 42% to 5.3% as estimated from two control postheparin plasma samples. The lipoprotein lipase calibration system is an easy and very cheap arrangement, which makes it possible to compare lipoprotein lipase activities achieved over years. When the lipoprotein lipase control values are compared with reference lipoprotein lipase samples determined in other lipase laboratories, the calibration-control system becomes an important tool for reducing analytical bias. The article reviews the original analytical criteria of catalytic measurement of lipoprotein lipase activity and describes the implementation of the calibration-control system. We describe a model for reduction of the analytical variability in the measurement of lipoprotein lipase activity. Other standardization efforts needto be made in the future, especially to define the reference material for calibration.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 01:47:38