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Titolo:
Effect of cocaine on cell proliferation in the cerebral wall of monkey fetuses
Autore:
Lidow, MS; Song, ZM;
Indirizzi:
Univ Maryland, Dept OCBS, Baltimore, MD 21201 USA Univ Maryland BaltimoreMD USA 21201 , Dept OCBS, Baltimore, MD 21201 USA Univ Maryland, Program Neurosci, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 am Neurosci, Baltimore, MD 21201 USA
Titolo Testata:
CEREBRAL CORTEX
fascicolo: 6, volume: 11, anno: 2001,
pagine: 545 - 551
SICI:
1047-3211(200106)11:6<545:EOCOCP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-SYNTHESIS; INTRAVENOUS COCAINE; PRENATAL EXPOSURE; INFANT OUTCOMES; RHESUS-MONKEY; RAT-BRAIN; IN-VITRO; CORTEX; RECEPTORS; PREGNANCY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Lidow, MS Univ Maryland, Dept OCBS, 5-A-12,HHH,666 W Baltimore St, Baltimore, MD 21201 USA Univ Maryland 5-A-12,HHH,666 W Baltimore St Baltimore MD USA 21201
Citazione:
M.S. Lidow e Z.M. Song, "Effect of cocaine on cell proliferation in the cerebral wall of monkey fetuses", CEREB CORT, 11(6), 2001, pp. 545-551

Abstract

This study examined the effect of cocaine on cell proliferation in the fetal monkey cerebral wall. Pregnant monkeys received cocaine daily (10 mg/kg,orally, in fruit treats, at 07.00 h and 19.00 h) beginning on the 40th dayof pregnancy (E40). The control animals received fruit treats only. One set of monkeys was used to examine the state of cell proliferation in the fetal cerebral wall at peak cocaine levels. These animals were injected with [H-3]thymidine intravenously on E73, 1.5 h after the morning drug or placeboadministration. Another set of monkeys was used to determine the state of cell proliferation after cocaine concentration declined to ineffective levels. These animals were injected with [3H]thymidine on the same day of pregnancy 10 h after the treatment, Cesarean sections were performed 40 min after the radioisotope injection, The right hemispheres were processed for autoradiography. The left hemispheres were used for biochemical analysis of theradioisotope incorporation into DNA. The third set of monkeys was used to determine whether chronic cocaine treatment extends the timing of neocortical neuronogenesis, These monkeys received their final cocaine treatment on E102 (the last day of normal neocortical neuronogenesis) and were injected with [3H]thymidine 24 h later. On E113, the fetal brains were processed foremulsion autoradiography. We found a significant decrease in the density of [H-3]thymidine-labeled cells and in the levels of this radioisotope incorporation into DNA in the fetal cerebral wall 1.5 h after cocaine administration. In contrast, 10 h after cocaine administration we detected a significantly elevated density of radiolabeled cells, and abnormally high levels of[3H]thymidine incorporation into DNA, This suggests that chronic intermittent administration of cocaine results in significant periodic fluctuations in cell production within the fetal cortical proliferative zones, We detected no cocaine-induced extension in neocortical neuronogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:48:13