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Titolo:
Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA
Autore:
Ohki, I; Shimotake, N; Fujita, N; Jee, JG; Ikegami, T; Nakao, M; Shirakawa, M;
Indirizzi:
Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan Nara Inst Sci & Technol Nara Japan 6300101 Biol Sci, Nara 6300101, Japan Yokohama City Univ, Grad Sch Integrated Sci, Kanagawa 2300045, Japan Yokohama City Univ Kanagawa Japan 2300045 d Sci, Kanagawa 2300045, Japan Kumamoto Univ, Sch Med, Dept Tumor Genet & Biol, Kumamoto 8600811, Japan Kumamoto Univ Kumamoto Japan 8600811 net & Biol, Kumamoto 8600811, Japan
Titolo Testata:
CELL
fascicolo: 4, volume: 105, anno: 2001,
pagine: 487 - 497
SICI:
0092-8674(20010518)105:4<487:SSOTMB>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTIONAL REPRESSOR MECP2; HISTONE DEACETYLASE COMPLEX; RETT-SYNDROME MUTATIONS; PROTEIN MBD1; ASSOCIATION; NUCLEOSOME; GENE; NMR; METHYLTRANSFERASE; CHROMATIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Shirakawa, M Nara Inst Sci & Technol, Grad Sch Biol Sci, 8916-5 Takayama, Nara 6300101,Japan Nara Inst Sci & Technol 8916-5 Takayama Nara Japan 6300101 n
Citazione:
I. Ohki et al., "Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA", CELL, 105(4), 2001, pp. 487-497

Abstract

In vertebrates, the biological consequences of DNA methylation are often mediated by protein factors containing conserved methyl-CpG binding domains (MBDs). Mutations in the MBD protein MeCP2 cause the neurodevelopmental disease Rett syndrome. We report here the solution structure of the MBD of thehuman methylation-dependent transcriptional regulator MBD1 bound to methylated BRA. DNA binding causes a loop in MBD1 to fold into a major and novel DNA binding interface. Recognition of the methyl groups and CG sequence at the methylation site is due to five highly conserved residues that form a hydrophobic patch. The structure indicates how MBD may access nucleosomal DNA without encountering steric interference from core histones, and providesa basis to interpret mutations linked to Rett syndrome in MeCP2.

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Documento generato il 20/01/20 alle ore 07:24:08