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Titolo:
Expression of Bax and Bcl-2 proteins during hypoxia in cerebral cortical neuronal nuclei of newborn piglets: effect of administration of magnesium sulfate
Autore:
Ravishankar, S; Ashraf, QM; Fritz, K; Mishra, OP; Delivoria-Papadopoulos, M;
Indirizzi:
Med Coll Penn & Hahnemann Univ, St Christophers Hosp Children, Div Neonatol, Dept Pediat, Philadelphia, PA USA Med Coll Penn & Hahnemann Univ Philadelphia PA USA Philadelphia, PA USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 901, anno: 2001,
pagine: 23 - 29
SICI:
0006-8993(20010518)901:1-2<23:EOBABP>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; GUINEA-PIG BRAIN; TRANSIENT FOREBRAIN ISCHEMIA; FREE RADICAL GENERATION; IN-UTERO HYPOXIA; GERBIL HIPPOCAMPUS; RAT-BRAIN; DNA FRAGMENTATION; GLOBAL-ISCHEMIA; UP-REGULATION;
Keywords:
Bax; Bcl-2; hypoxia; magnesium; apoptosis; newborn brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Ravishankar, S Med Coll Penn & Hahnemann Univ, Div Neonatol Res, 3300 Henry Ave,Room 701,7th Floor, Philadelphia, PA 19129 USA Med Coll Penn & Hahnemann Univ 3300 Henry Ave,Room 701,7th Floor Philadelphia PA USA 19129
Citazione:
S. Ravishankar et al., "Expression of Bax and Bcl-2 proteins during hypoxia in cerebral cortical neuronal nuclei of newborn piglets: effect of administration of magnesium sulfate", BRAIN RES, 901(1-2), 2001, pp. 23-29

Abstract

This study tests the hypothesis that administration of magnesium sulfate, an antagonist of the NMDA receptor ion-channel. will prevent the hypoxia-induced alteration in the expression and the ratio of Bax and Bcl-2 proteins in cerebral cortical neuronal nuclear membranes. Anesthetized. ventilated and instrumented newborn piglets were divided into three groups: normoxic controls (Nx), untreated hypoxic (Hx), and magnesium sulfate-treated hypoxic (Mg-Hx) groups. Cerebral hypoxia was induced by lowering the FiO(2) (0.05-0.07) for 1 h and the cerebral cortex was harvested immediately for isolation of neuronal nuclei and hypoxia was confirmed biochemically by a decrease in the tissue levels of ATP and phosphocreatine (PCr). Brain tissue PCr (mumol/g brain) was 2.74+/-0.77 (Nx), 0.38+/-0.09 (Hx, P<0.05 vs. Nx) and 0.69+/-0.60 (Mg-Hx, P<0.05 vs. Nx). The density of immunoblotted proteins was expressed as absorbance (Axmm(2)). The expression of Bax protein (Axmm(2)) was 222+/-31 (Nx), 279+/-32 (Hx), and 148+/-44 (Mg-Hx, P<0.05 vs. Hx). Bcl-2 protein expression was 77+/-1.0 (Nx), 37+/-5.0 (Hx) and 46+/-15 (Mg-Hx, P<0.05 vs. Nx). The ratio of Bax to Bcl-2 proteins increased more than twofold during hypoxia as compared to normoxia (7:1 Hx vs. 3:1 Nx). However. in the magnesium sulfate-treated group the Bax:Bcl-2 ratio was similar to normoxic controls. The data demonstrate that magnesium sulfate treatment prevents both the hypoxia-induced increase in Bax protein expression and the alteration of Bax:Bcl-2 protein ratios. We suggest that magnesium sulfate treatment before and during hypoxia may decrease hypoxia-induced programmed celldeath by maintaining the normal ratio of Bax to Bcl-2 proteins. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 13:29:13