Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Characterization of soluble protein extracts from keratinized tissues: Identification of ubiquitin universally distributed in hair, nail, and stratumcorneum
Autore:
Inoue, T; Kizawa, K; Ito, M;
Indirizzi:
Kanebo Ltd, Basic Res Labs, Odawara, Kanagawa 2500002, Japan Kanebo Ltd Odawara Kanagawa Japan 2500002 dawara, Kanagawa 2500002, Japan
Titolo Testata:
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
fascicolo: 4, volume: 65, anno: 2001,
pagine: 895 - 900
SICI:
0916-8451(200104)65:4<895:COSPEF>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALCIUM-BINDING PROTEIN; CYSTEINE-RICH; S100A3; LOCALIZATION; CUTICLE;
Keywords:
ubiquitin; S100A3; hair; nail; stratum corneum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, T Kanebo Ltd, Basic Res Labs, Kotobuki Cho 5-3-28, Odawara, Kanagawa 2500002, Japan Kanebo Ltd Kotobuki Cho 5-3-28 Odawara Kanagawa Japan 2500002 pan
Citazione:
T. Inoue et al., "Characterization of soluble protein extracts from keratinized tissues: Identification of ubiquitin universally distributed in hair, nail, and stratumcorneum", BIOS BIOT B, 65(4), 2001, pp. 895-900

Abstract

Partial protein extracts were prepared from hair, nail, and stratum corneum in the absence of urea and Interfacial surfactant. Tricine-sodium dodecylsulfate polyacrylamide gel electrophoreses of these extracts showed low-molecular tl eight protein-rich patterns apparently different from those of whole protein extracts, which mainly consist of keratin bands. Several protein bands characterized each keratinized tissue or its derived species. In addition, we identified a major band of approximately 7 kDa as ubiquitin, a ubiquitously distributed protein that mediates: non-lysosomal protein degradation, through direct amino acid sequence analysis of the electro-blotted protein band. The partial extraction is useful for investigation of solubleproteins retained in tile keratinized tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 16:26:34