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Titolo:
Co-expression of multiple transgenes in mouse CNS: a comparison of strategies
Autore:
Jankowsky, JL; Slunt, HH; Ratovitski, T; Jenkins, NA; Copeland, NG; Borchelt, DR;
Indirizzi:
Johns Hopkins Sch Medt, Dept Pathol, Baltimore, MD 21205 USA Johns HopkinsSch Medt Baltimore MD USA 21205 ol, Baltimore, MD 21205 USA Johns Hopkins Sch Medt, Dept Neurosci, Baltimore, MD 21205 USA Johns Hopkins Sch Medt Baltimore MD USA 21205 ci, Baltimore, MD 21205 USA NCI, Mouse Canc Genet Program, Frederick Canc Res & Dev Ctr, Frederick, MD21702 USA NCI Frederick MD USA 21702 ick Canc Res & Dev Ctr, Frederick, MD21702 USA
Titolo Testata:
BIOMOLECULAR ENGINEERING
fascicolo: 6, volume: 17, anno: 2001,
pagine: 157 - 165
SICI:
1389-0344(200106)17:6<157:COMTIM>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYLOID PRECURSOR PROTEIN; ALZHEIMERS-DISEASE; IN-VIVO; NUCLEAR TRANSFER; MICE; PRESENILIN-1; IDENTIFICATION; RESCUES; BRAINS; PS1;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Borchelt, DR Johns Hopkins Sch Medt, Dept Pathol, 720 Rutland Ave,558 RossRes Bldg, Baltimore, MD 21205 USA Johns Hopkins Sch Medt 720 Rutland Ave,558 Ross Res Bldg Baltimore MD USA 21205
Citazione:
J.L. Jankowsky et al., "Co-expression of multiple transgenes in mouse CNS: a comparison of strategies", BIOMOL ENG, 17(6), 2001, pp. 157-165

Abstract

The introduction of two transgenes into one animal is increasingly common as transgenic experiments become more sophisticated. In this study we examine two strategies for creating double transgenic founders from a single microinjection. In the first approach, two constructs, each with its own promoter element, were coinjected into the pronucleus. In the second approach, both transgenes were cloned into one vector, separated by an internal ribosomal entry site (IRES), and placed under control of a single promoter. Both strategies save time and increase the percentage of double transgenic offspring over the standard method of mating single transgenic lines. However, despite high transgene copy numbers, the bicistronic lines did not show robust expression of either protein. Copy number and protein expression correlated much better in the coinjected lines, with expression levels in one lineapproaching that observed in some of our best single transgenic controls. Thus we recommend coinjection of individual plasmids for the generation of multiply transgenic founders. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:32:58