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Titolo:
Cloning and characterization of the Pseudomonas sp 61-3 phaG gene involvedin polyhydroxyalkanoate biosynthesis
Autore:
Matsumoto, K; Matsusaki, H; Taguchi, S; Seki, M; Doi, Y;
Indirizzi:
RIKEN Inst, Polymer Chem Lab, Wako, Saitama 3500198, Japan RIKEN Inst Wako Saitama Japan 3500198 m Lab, Wako, Saitama 3500198, Japan Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan Univ Tokyo Tokyo Japan 1138656 otechnol, Bunkyo Ku, Tokyo 1138656, Japan
Titolo Testata:
BIOMACROMOLECULES
fascicolo: 1, volume: 2, anno: 2001,
pagine: 142 - 147
SICI:
1525-7797(200121)2:1<142:CACOTP>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHAIN-LENGTH CONSTITUENTS; NONRELATED CARBON-SOURCES; GRAM-NEGATIVE BACTERIA; AEROMONAS-CAVIAE; HYDRATASE GENES; COPOLYESTER; SP-61-3; ACIDS; DERIVATIVES; MUTAGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Doi, Y RIKEN Inst, Polymer Chem Lab, 2-1 Hirosawa, Wako, Saitama 3500198, Japan RIKEN Inst 2-1 Hirosawa Wako Saitama Japan 3500198 a 3500198, Japan
Citazione:
K. Matsumoto et al., "Cloning and characterization of the Pseudomonas sp 61-3 phaG gene involvedin polyhydroxyalkanoate biosynthesis", BIOMACROMOL, 2(1), 2001, pp. 142-147

Abstract

Pseudomonas sp. 61-3 produces a blend of poly(3-hydroxybutyrate) [P(3HB)] homopolymer and poly(3-whydroxybutyrate-co-3-hydroxyalkanoates) [P(3HB-co-3HA)] random copolymer consisting of monomeric units of 4-12 carbon atoms from sugars. The phaG(Ps) gene encoding (R)-3-hydroxyacyl-acyl carrier protein coenzyme A transferase was cloned from this strain, and homologous expression of this gene under the control of the lac or the native promoter was investigated. Additional copies of the phaG(Ps), gene in Pseudomonas sp. 61-3 led to an increase in both the polyhydroxyalkanoate (PHA) content in the cells and the fraction of medium-chain-length 3HA units in PHA. Disruption of the chromosomal phaG(Ps) gene resulted in an increase in the fraction ofthe 3HB unit in PHA. The site-directed mutagenesis of the phaG(Ps) gene was carried out to investigate the role of a HX4D motif which has been proposed to be related to PhaG activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:35:35