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Titolo:
Nanoscale clustering of RGD peptides at surfaces using comb polymers. 1. Synthesis and characterization of comb thin films
Autore:
Irvine, DJ; Mayes, AM; Griffith, LG;
Indirizzi:
MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139 , Dept Mat Sci & Engn, Cambridge, MA 02139 USA MIT, Dept Chem Engn, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139MIT, Dept Chem Engn, Cambridge, MA 02139 USA MIT, Div Bioengn & Environm Hlth, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139 oengn & Environm Hlth, Cambridge, MA 02139 USA
Titolo Testata:
BIOMACROMOLECULES
fascicolo: 1, volume: 2, anno: 2001,
pagine: 85 - 94
SICI:
1525-7797(200121)2:1<85:NCORPA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; POLY(ETHYLENE OXIDE); FOCAL ADHESIONS; CELL-ADHESION; ADSORPTION; BIOMATERIALS; CYTOSKELETAL; COPOLYMERS; ACTIVATION; MOLECULES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Mayes, AM MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA MIT CambridgeMA USA 02139 Sci & Engn, Cambridge, MA 02139 USA
Citazione:
D.J. Irvine et al., "Nanoscale clustering of RGD peptides at surfaces using comb polymers. 1. Synthesis and characterization of comb thin films", BIOMACROMOL, 2(1), 2001, pp. 85-94

Abstract

Theoretical and experimental studies were conducted to elucidate the structure and properties of amphiphilic comb polymer thin films presenting nanoscale clusters of Arg-Gly-Asp (RGD) peptides for control of cell adhesion onbiomaterials. Combs comprised of a poly(methyl methacrylate) backbone and short poly(ethylene oxide) side chains were synthesized, and peptides were tethered to the side chain ends to create nanoscale peptide clusters. In thin films, comb polymers containing greater than or equal to 30 wt % six to nine unit PEO side chains completely resisted adhesion of a model fibroblast cell line in the presence of 7.5% serum over 24 h. These same polymers modified with RGD peptides elicited tunable cell adhesion when mixed with unmodified combs in varying proportion. A self-consistent field lattice model of the interface between comb polymer films and water predicts an organization of the top molecular layer of comb polymer with the backbone oriented parallel to the interface in quasi-two-dimensional confinement and hydrophilic side chains extended in a brushlike layer into solution. This picture ofa quasi-2D configuration is consistent with the observed surface properties of comb films in water as well as measurements of the RGD cluster densityon mixed comb surfaces using fluorescent nanosphere labeling of ligand clusters.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 11:55:50