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Titolo:
Measurement of copper-binding sites on low density lipoprotein
Autore:
Roland, A; Patterson, RA; Leake, DS;
Indirizzi:
Univ Reading, Sch Anim & Microbial Sci, Cell & Mol Biol Res Div, Reading RG6 5AJ, Berks, England Univ Reading Reading Berks England RG6 5AJ eading RG6 5AJ, Berks, England
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 4, volume: 21, anno: 2001,
pagine: 594 - 602
SICI:
1079-5642(200104)21:4<594:MOCSOL>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ATHEROSCLEROTIC LESIONS; CORONARY-HEART-DISEASE; ACTIVATING-FACTOR ACETYLHYDROLASE; TRANSITION-METAL IONS; OXIDATIVE MODIFICATION; LIPID-PEROXIDATION; FLAVONOID INTAKE; LDL OXIDATION; ANTIOXIDANT PROPERTIES; CATALYZED OXIDATION;
Keywords:
low density lipoproteins; atherosclerosis; copper; flavonoids; quercetin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
74
Recensione:
Indirizzi per estratti:
Indirizzo: Roland, A Univ Reading, Sch Anim & Microbial Sci, Cell & Mol Biol Res Div,POB 228, Reading RG6 5AJ, Berks, England Univ Reading POB 228 Reading Berks England RG6 5AJ erks, England
Citazione:
A. Roland et al., "Measurement of copper-binding sites on low density lipoprotein", ART THROM V, 21(4), 2001, pp. 594-602

Abstract

Copper is often used to oxidize low density lipoprotein (LDL) in experiments in vitro and is a candidate for oxidizing LDL in atherosclerotic lesions. The binding of copper ions to LDL is usually thought to be a prerequisitefor LDL oxidation by copper, although estimates of LDL copper binding varywidely. We have developed and validated an equilibrium dialysis assay in aMOPS-buffered system to measure copper binding to LDL and have found 38.6 /- 0.7 (mean +/- SEM, n=25) copper binding sites on LDL. The binding was saturated at a copper concentration of 10 mu mol/L at LDL concentrations of up to 1 mg protein/mL. Copper-binding capacity increased progressively and markedly when LDL was oxidized to increasing extents. Chemical modificationof histidyl and lysyl residues on apolipoprotein B-100 reduced the number of binding sites by 56% and 23%, respectively. As an example of the potential of this method to assess the effects of antioxidants on copper binding to LDL, we have shown that the flavonoids myricetin, quercetin, and catechin(but not epicatechin, kaempferol, or morin), at concentrations equimolar to the copper present (10 mu mol/L), significantly decreased copper binding to LDL by 82%, 56%, and 20%, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:18:14