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Titolo:
Mast cell chymase induces apoptosis of vascular smooth muscle cells
Autore:
Leskinen, M; Wang, YF; Leszczynski, D; Lindstedt, KA; Kovanen, PT;
Indirizzi:
Wihuri Res Inst, SF-00140 Helsinki, Finland Wihuri Res Inst Helsinki Finland SF-00140 st, SF-00140 Helsinki, Finland STUK Radiat & Nucl Safety Author, Helsinki, Finland STUK Radiat & Nucl Safety Author Helsinki Finland or, Helsinki, Finland
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 4, volume: 21, anno: 2001,
pagine: 516 - 522
SICI:
1079-5642(200104)21:4<516:MCCIAO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ATHEROSCLEROTIC PLAQUES; PLASMA PROTEINASE-INHIBITORS; SHOULDER REGION; KINASE-C; HEPARIN; RUPTURE; PROTEASES; ATHEROMA; PROTEOGLYCANS; ACCUMULATION;
Keywords:
apoptosis; smooth muscle; atherosclerosis; mast cells; chymase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Kovanen, PT Wihuri Res Inst, Kalliolinnantie 4, SF-00140 Helsinki, FinlandWihuri Res Inst Kalliolinnantie 4 Helsinki Finland SF-00140 d
Citazione:
M. Leskinen et al., "Mast cell chymase induces apoptosis of vascular smooth muscle cells", ART THROM V, 21(4), 2001, pp. 516-522

Abstract

In human coronary atheromas, the numbers of degranulated mast cells and ofapoptotic smooth muscle cells (SMCs) are increased. Accordingly, the possibility exists that mast cells participate in the regulation of SMC apoptosis in the lesions. Mast cells isolated from the serosal cavities of rats were stimulated to release their secretory granules. The neutral protease chymase, present in the exocytosed granules, was found to induce apoptosis whenadded to rat aortic SMCs in culture. The chymase-induced apoptosis of SMCswas detected by flow cytometry, microscopic analysis of cellular morphology, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL), and electrophoretic demonstration of DNA laddering. Chymase induced SMC apoptosis in a dose- and time-dependent manner, and its proteolytic activity was essential for the proapoptotic effect. In addition to rat chymase, recombinant human chymase was also found to induce apoptosis of human coronary artery SMCs in culture. These results suggest that mast cells may participate in the apoptotic regulation of SMCs in atherosclerotic lesions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 20:52:35