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Titolo:
Use of sulfate production as a measure of short-term sulfur amino acid catabolism in humans
Autore:
Hamadeh, MJ; Hoffer, LJ;
Indirizzi:
McGill Univ, Jewish Gen Hosp, Lady Davis Inst Med Res, Montreal, PQ H3T 1E2, Canada McGill Univ Montreal PQ Canada H3T 1E2 Res, Montreal, PQ H3T 1E2, Canada McGill Univ, Sch Dietet & Human Nutr, St Anne De Bellevue, PQ H9X 3V9, Canada McGill Univ St Anne De Bellevue PQ Canada H9X 3V9 vue, PQ H9X 3V9, Canada
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
fascicolo: 6, volume: 280, anno: 2001,
pagine: E857 - E866
SICI:
0193-1849(200106)280:6<E857:UOSPAA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
BODY LEUCINE KINETICS; INORGANIC SULFATE; UREA PRODUCTION; METABOLISM; PROTEIN; ABSORPTION; EXCRETION; WATER; GLUTATHIONE; GENERATION;
Keywords:
stable isotope; amino acid oxidation; methionine; fed state;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffer, LJ McGill Univ, Jewish Gen Hosp, Lady Davis Inst Med Res, 3755 Cote Ste Catherine Rd, Montreal, PQ H3T 1E2, Canada McGill Univ 3755 Cote Ste Catherine Rd Montreal PQ Canada H3T 1E2
Citazione:
M.J. Hamadeh e L.J. Hoffer, "Use of sulfate production as a measure of short-term sulfur amino acid catabolism in humans", AM J P-ENDO, 280(6), 2001, pp. E857-E866

Abstract

There is no fully satisfactory method for measuring amino acid catabolism in the nonsteady state that follows normal protein consumption. Because sulfate is the major product of sulfur amino acid catabolism, we tested whether its production can be accurately depicted using simple tracer or nontracer approaches under basal conditions and after the intravenous administration of a known amount of sulfate. In the basal postabsorptive state, serum sulfate concentration and urinary sulfate excretion remained constant for many hours, but the apparent steady-state serum sulfate rate of appearance achieved with primed continuous oral administration of sodium [S-34]sulfate was 20% higher than urinary sulfate excretion. By contrast, after magnesium sulfate infusion, the increase in sulfate production above basal accounted for 95% over 6 h and 98% over 9 h of the administered dose when measured simply as urinary inorganic sulfate excretion corrected for changes in its extracellular fluid content. Using the latter method, we measured sulfate production after oral methionine and intravenous infusion of methionine in a mixture of other essential amino acids. Sulfate production above basal accounted for 59% over 6 h and 75% over 9 h of the oral methionine dose. Similar results were obtained with the mixed amino acid infusion, but interpretation of the latter experiment was limited by the mild protein sparing (and, hence, reduced endogenous sulfate production) induced by the amino acid infusion. We conclude that a simple nontracer method can provide an accurate measure of sulfate production and, hence, sulfur amino acid catabolism over collection periods as short as 6 h after a test meal. A significant portion of the sulfur derived from methionine appears to be retained in nonprotein compounds immediately after its ingestion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:27:32