Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Energy-related metabolites during and after induced myocardial infarction with special emphasis on the reperfusion injury after extracorporeal circulation
Autore:
Zemgulis, V; Ronquist, G; Bjerner, T; Henze, A; Waldenstrom, A; Thelin, S; Wikstrom, G;
Indirizzi:
Univ Uppsala Hosp, Dept Cardiothorac Surg, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 c Surg, S-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Clin Chem, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 n Chem, S-75185 Uppsala, Sweden Univ Uppsala Hosp, Dept Diagnost Radiol, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 Radiol, S-75185 Uppsala, Sweden Univ Umea Hosp, Dept Internal Med, S-90185 Umea, Sweden Univ Umea Hosp Umea Sweden S-90185 pt Internal Med, S-90185 Umea, Sweden Univ Uppsala Hosp, Dept Cardiol, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 ardiol, S-75185 Uppsala, Sweden
Titolo Testata:
ACTA PHYSIOLOGICA SCANDINAVICA
fascicolo: 2, volume: 171, anno: 2001,
pagine: 129 - 143
SICI:
0001-6772(200102)171:2<129:EMDAAI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACIDS; CORONARY-ARTERY; ISCHEMIC MYOCARDIUM; INTERSTITIAL FLUID; GENE-EXPRESSION; BYPASS-SURGERY; CONSCIOUS DOG; ANIMAL-MODELS; HEART; PEROXYNITRITE;
Keywords:
cardioplegia; energy metabolism; ischaemia; microdialysis; pig; reperfusion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Zemgulis, V Univ Uppsala Hosp, Dept Cardiothorac Surg, S-75185 Uppsala, Sweden Univ Uppsala Hosp Uppsala Sweden S-75185 185 Uppsala, Sweden
Citazione:
V. Zemgulis et al., "Energy-related metabolites during and after induced myocardial infarction with special emphasis on the reperfusion injury after extracorporeal circulation", ACT PHYSL S, 171(2), 2001, pp. 129-143

Abstract

In the clinical setting great efforts have been made with contradictory results to operate upon acutely myocardial ischaemic patients. The reasons for the absence of clear-cut results are not well understood nor are they scientifically explored. To resolve this problem further, we attempted to design an experimental in vivo model to mimic acute myocardial ischaemia followed by extracorporeal circulation (ECC) and reperfusion. One of the main targets of our protocol was monitoring of myocardial energy metabolism by microdialysis (MCD) during the periods of coronary occlusion (60 min), hypothermic (30 degreesC) ECC and cardioplegia (45 min), followed by reperfusion with (30 min) and without (60 min) ECC. In eight anaesthetized, open-chest pigs, myocardial lactate, pyruvate, adenosine, taurine, inosine, hypoxanthineand guanosine were sampled with MCD in both ischaemic and non-ischaemic areas. Myocardial area at risk and infarct size were quantified with the modified topographical evaluation methods. The principal finding with this experimental setup was a biphasic release pattern of lactate, adenosine, taurine, inosine, hypoxanthine and guanosine from ischaemic myocardium. Lactate levels were equally high in reperfused ischaemic and non-ischaemic myocardial tissue. Pyruvate demonstrated consistently higher values in non-ischaemicmyocardium throughout the experiment. A pattern was discernible, lactate being a marker of compromised cell energy metabolism, and taurine being a marker of disturbed cell integrity. Of special interest was the increased level of pyruvate in microdialysates of non-ischaemic myocardium as compared with its ischaemic counterpart. In conclusion, we found disturbances in energy metabolism and cell integrity not only in ischaemic but also in non-ischaemic tissue during reperfusion implying that non-ischaemic myocardium demonstrated an unexpected accumulation of lactate and pyruvate. These new findings could at least partly be explicatory to the increased risk of heart surgery in connection with acute myocardial infarction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:43:27