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Titolo:
Long-term graft acceptance in rat heart transplantation by CTLA4Ig gene transfection combined with FTY720 treatment
Autore:
Ohba, M; Li, XK; Kita, Y; Enosawa, S; Funeshima, N; Nagai, H; Zhang, HQ; Okuyama, T; Ogoshi, S; Sasaguri, S; Amemiya, H; Suzuki, S;
Indirizzi:
Natl Childrens Med Res Ctr, Dept Exptl Surg & Bioengn, Setagaya Ku, Tokyo 1548509, Japan Natl Childrens Med Res Ctr Tokyo Japan 1548509 Ku, Tokyo 1548509, Japan Kochi Med Sch, Dept Surg 2, Nanko Ku, Kochi 7838505, Japan Kochi Med Sch Kochi Japan 7838505 Surg 2, Nanko Ku, Kochi 7838505, Japan Natl Childrens Med Res Ctr, Dept Genet, Setagaya Ku, Tokyo 1548509, Japan Natl Childrens Med Res Ctr Tokyo Japan 1548509 Ku, Tokyo 1548509, Japan
Titolo Testata:
WORLD JOURNAL OF SURGERY
fascicolo: 4, volume: 25, anno: 2001,
pagine: 391 - 398
SICI:
0364-2313(200104)25:4<391:LGAIRH>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT ADENOVIRUSES IMPROVES; MHC CLASS-I; ALLOGRAFT-REJECTION; E1-DELETED ADENOVIRUSES; TRANSGENE EXPRESSION; LYMPHOCYTE APOPTOSIS; CARDIAC ALLOGRAFTS; MOUSE HEPATOCYTES; LIVER ALLOGRAFTS; CELL ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, S Natl Childrens Med Res Ctr, Dept Exptl Surg & Bioengn, Setagaya Ku, 3-35-31 Taishido, Tokyo 1548509, Japan Natl Childrens Med Res Ctr 3-35-31 Taishido Tokyo Japan 1548509
Citazione:
M. Ohba et al., "Long-term graft acceptance in rat heart transplantation by CTLA4Ig gene transfection combined with FTY720 treatment", WORLD J SUR, 25(4), 2001, pp. 391-398

Abstract

CTLA4Ig strongly adheres to B7 molecules on antigen-presenting cells to block intracellular signal transduction via CD28 an helper T cells, which eventually inhibits immune responses. We have demonstrated that the administration to recipient animals of adenoviral vectors containing CTLA4Ig gene (adCTLA4Ig) prolonged graft survival, although the gene expression diminished in a time-dependent manner and the grafts were finally rejected. In addition, recipient animals treated with FTY720, a new immunosuppressant, exhibited a decrease in the number of peripheral lymphocytes due to apoptosis. In this study, we performed adCTLA4Ig transfection combined with FTY720 treatment in heart-grafted rats to determine if the combination could induce a mutual effect on graft survival. The recipient animals were given injections of 1 x 10(9) plaque-forming units of adCTLA4Ig via the tail vein immediatelyafter grafting. On the day before transplantation we administered FTY720 orally to some of these animals at a dosage of 5 mg/kg and again on the day of transplantation. The median graft survival period in the adCTLA4Ig-only group was 27 days, whereas that in the combination group was markedly prolonged to 56 dags. Of 15 grafts, 5 survived indefinitely. In these groups we observed detectable levels of CTLA4Ig in the sera 49 days after grafting; the levels were always higher in the combination group than in the adCTLA4Ig-only group. As a result, this study revealed that FTY720 and adCTLA4Ig have a potent mutual effect on graft survival during rat heart transplantation. Furthermore, it is: highly possible that FTY720 enhances gene expression of adCTLA4Ig, which map be related to the long-term acceptance of grafts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:57:26