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Titolo:
Simian retrovirus vectors for gene transfer in nonhuman primate cells
Autore:
Li, B; Nguyen, S; Li, XR; Machida, CA;
Indirizzi:
Oregon Hlth Sci Univ, Oregon Reg Primate Res Ctr, Div Neurosci, Beaverton,OR 97006 USA Oregon Hlth Sci Univ Beaverton OR USA 97006 rosci, Beaverton,OR 97006 USA Oregon Hlth Sci Univ, Dept Biochem & Mol Biol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 l Biol, Portland, OR 97201 USA Oregon Hlth Sci Univ, Grad Program Neurosci, Portland, OR 97201 USA OregonHlth Sci Univ Portland OR USA 97201 urosci, Portland, OR 97201 USA
Titolo Testata:
VIRUS RESEARCH
fascicolo: 2, volume: 75, anno: 2001,
pagine: 155 - 168
SICI:
0168-1702(200106)75:2<155:SRVFGT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATION-COMPETENT RETROVIRUS; MAMMALIAN-CELLS; NUCLEOTIDE-SEQUENCE; GENERATION; VIRUS; LINE; RECOMBINATION; SAFE;
Keywords:
simian retrovirus (SRV) vectors; D2/RHE/OR/V1 serogroup 2 SRV; psi packaging signal; split-genome packaging cell recombinants; SRV-based gene transfer system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Machida, CA Oregon Hlth Sci Univ, Oregon Reg Primate Res Ctr, Div Neurosci, Univ E Campus,505 NW 185th Ave, Beaverton, OR 97006 USA Oregon Hlth Sci Univ Univ E Campus,505 NW 185th Ave Beaverton OR USA 97006
Citazione:
B. Li et al., "Simian retrovirus vectors for gene transfer in nonhuman primate cells", VIRUS RES, 75(2), 2001, pp. 155-168

Abstract

We have recently identified and sequenced a molecular clone of the serogroup 2 simian retrovirus (SRV), D2/RHE/OR/V1, that retains an enhanced ability to infect specific T cell lines. In this report, using deletion mutagenesis, we localized the psi packaging signal, necessary for packaging of D2/RHE/OR/V1 particles, to the genomic region 345-650, which comprises the 5' intergenic region (IR) and the extreme 5' portion of the gag gene. To build an SRV-based gene transfer system and to reduce the possibility of recombination and regeneration of replication-competent viruses, we constructed split-genome D2/RHE/OR/V1 plasmid recombinants containing distinct and non-overlapping retroviral gene regions and several replacement components. For theretrovirus gene transfer vehicle, we deleted the D2/RHE/OR/V1 structural genes and substituted a cassette including the psi-packaging region, the P-galactosidase reporter gene, and the 3' IR. Both packaging cell recombinantswere used to generate stable monkey packaging cell lines; the gene transfer vehicle was subsequently transfected into the packaging cell lines, and replication-defective viruses were recovered for subsequent infection into fresh monkey cells. Successful infection by the recovered viruses verifies the potential efficacy of the SRV-based system as a research tool for gene transfer of heterologous genes into nonhuman primate cells. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 05/04/20 alle ore 22:38:37