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Titolo:
Immunostimulatory sequence up-modulate the interaction between immunoglobulins and plasmid DNA at acidic pH
Autore:
Hu, ZL; Sun, SH; Dai, JX; Zhou, FJ;
Indirizzi:
Second Mil Med Univ, Dept Med Genet, Shanghai 200433, Peoples R China Second Mil Med Univ Shanghai Peoples R China 200433 433, Peoples R China
Titolo Testata:
VACCINE
fascicolo: 23-24, volume: 19, anno: 2001,
pagine: 3051 - 3057
SICI:
0264-410X(20010430)19:23-24<3051:ISUTIB>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
BACTERIAL-DNA; CPG MOTIFS; CELLS; ACTIVATION; BINDING; INTERNALIZATION; LEUKOCYTES; MECHANISM; INNATE;
Keywords:
immunostimulatory sequence; immunoglobulins; one-hybrid system;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Sun, SH Second Mil Med Univ, Dept Med Genet, Shanghai 200433, Peoples R China Second Mil Med Univ Shanghai Peoples R China 200433 ples R China
Citazione:
Z.L. Hu et al., "Immunostimulatory sequence up-modulate the interaction between immunoglobulins and plasmid DNA at acidic pH", VACCINE, 19(23-24), 2001, pp. 3051-3057

Abstract

To identify specific receptors for immunostimulatory sequence (ISS), a cDNA library of human bone marrow was screened by one-hybrid system using ISS as bait. Among four duel-positive clones isolated so far, two encode light chain of immunoglobulin (Ig) homologous to anti-DNA and anti-HBsAg (HBsAb),respectively, suggesting a direct interaction between ISS and Igs. ELISA revealed that HBsAb, as well as Igs in normal mouse serum and a monoclonal anti-v5 antibody, bound to plasmid DNA in a pH-dependent way; more importantly, the plasmids inserted with more copies of ISS shown higher reactivity with these Ifs than their blank vectors. Electrophoretic mobility shift assay (EMSA) showed HBsAb bound to oligonucleotides (ODN) also in a pi-I-dependent and ISS-specific way. Furthermore, ISS-containing ODN could enhance thebinding of Igs to plasmid DNA at acidic pH. These results support existence of direct ISS-Ig interaction and demonstrated that DNA-Ig interaction could be up-modulated by ISS. In consideration of the crucial role of membraneIE (mIg) in B cell activation, these results suggest mig may be a candidate ISS receptor in ISS-induced B cell activation. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:10:47