Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Inhibition of apoptosis in human neutrophils by Helicobacter pylori water-soluble surface proteins
Autore:
Kim, JS; Kim, JM; Jung, HC; Song, IS; Kim, CY;
Indirizzi:
Seoul Natl Univ, Coll Med, Dept Internal Med, Chongno Gu, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 o Gu, Seoul 110744, South Korea Seoul Natl Univ, Coll Med, Liver Res Inst, Seoul 151, South Korea Seoul Natl Univ Seoul South Korea 151 r Res Inst, Seoul 151, South Korea Hanyang Univ, Coll Med, Dept Microbiol, Seoul 133791, South Korea Hanyang Univ Seoul South Korea 133791 crobiol, Seoul 133791, South Korea Hanyang Univ, Coll Med, Inst Biomed Sci, Seoul 133791, South Korea HanyangUniv Seoul South Korea 133791 med Sci, Seoul 133791, South Korea
Titolo Testata:
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
fascicolo: 6, volume: 36, anno: 2001,
pagine: 589 - 600
SICI:
0036-5521(200106)36:6<589:IOAIHN>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR RECEPTOR; FAS LIGAND; CELL-DEATH; CHEMOTACTIC ACTIVITY; MEDIATED APOPTOSIS; EPITHELIAL-CELLS; DOWN-REGULATION; IN-VITRO; EXPRESSION; INFECTION;
Keywords:
Helicobacter pylori water extract; Fas; Fas ligand; neutrophil apoptosis; tumor necrosis; factor receptor 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Jung, HC Seoul Natl Univ, Coll Med, Dept Internal Med, Chongno Gu, 28 Yongon Dong, Seoul 110744, South Korea Seoul Natl Univ 28 Yongon Dong Seoul South Korea 110744 th Korea
Citazione:
J.S. Kim et al., "Inhibition of apoptosis in human neutrophils by Helicobacter pylori water-soluble surface proteins", SC J GASTR, 36(6), 2001, pp. 589-600

Abstract

Background: Helicobacter pylori infection in humans causes persistent neutrophil infiltration into the gastric mucosa. It is believed that a prolongation of neutrophil life-span could contribute to the pathogenesis of H. pylori infection. We therefore examined whether the water-soluble surface proteins of H. pylori can influence the apoptosis of neutrophils. Methods: After neutrophils were incubated with H. pylori water extract (HPWE), neutrophil apoptosis was evaluated by TUNEL, assay, Hoechst 33342 staining, electronmicroscopy and ELISA for cytosolic oligonucleosome-bound DNA for up to 48 h. To investigate the regulatory mechanisms of neutrophil apoptosis associated with HPWE, mRNA expression and protein production of Fas, Fas ligand (FasL) and tumor necrosis factor receptor 1 (TNF-R1) were analyzed by RT-PCR,ribonuclease protection assay, Northern blot and Western blotting. Cell surface expression of these death factors was also measured by flow cytometry. Results: HPWE inhibited neutrophil apoptosis and cytotoxicity for up to 48 h. The mRNA and protein expression of Fast and the cell surface expression of Fas, Fast and TNF-R1 in HPWE-treated neutrophils were suppressed compared with the controls. Conclusion: The water-soluble surface proteins of H.pylori could suppress neutrophil apoptosis. This may be caused by the suppression of Fast expression in neutrophils and Fas, Fast and TNF-R1 expression on the surface of neutrophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 19:08:54