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Titolo:
Possible role of kallikrein in proteolysis of insulin-like growth factor binding proteins during the oestrous cycle and early pregnancy in pigs
Autore:
Geisert, RD; Chamberlain, CS; Vonnahme, KA; Malayer, JR; Spicer, LJ;
Indirizzi:
Oklahoma State Univ, Oklahoma Agr Expt Stn, Dept Anim Sci, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 Sci, Stillwater, OK 74078 USA Oklahoma State Univ, Coll Vet Med, Dept Physiol Sci, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 Sci, Stillwater, OK 74078 USA
Titolo Testata:
REPRODUCTION
fascicolo: 5, volume: 121, anno: 2001,
pagine: 719 - 728
SICI:
1470-1626(200105)121:5<719:PROKIP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-I; CONCEPTUS DEVELOPMENT; PORCINE UTERUS; MESSENGER-RNA; ESTROUS-CYCLE; GENOMIC ORGANIZATION; UTERINE KALLIKREIN; FOLLICULAR-FLUID; RECEPTOR PROTEIN; GENE-EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Geisert, RD Oklahoma State Univ, Oklahoma Agr Expt Stn, Dept Anim Sci, Stillwater, OK 74078 USA Oklahoma State Univ Stillwater OK USA 74078 ter, OK 74078 USA
Citazione:
R.D. Geisert et al., "Possible role of kallikrein in proteolysis of insulin-like growth factor binding proteins during the oestrous cycle and early pregnancy in pigs", REPRODUCT, 121(5), 2001, pp. 719-728

Abstract

During early pregnancy, pig conceptuses initiate the synthesis of oestrogens and on day 12 their trophoblastic membranes undergo a rapid expansion throughout the uterine horns. The insulin-like growth factor (IGF) system maybe involved with conceptus development and steroidogenesis in pigs. Changes in uterine luminal ICF, insulin-like growth factor binding proteins (IGFBPs) and enzymatic activity for cleavage of IGFBPs during the oestrous cycleand early pregnancy were investigated. Uterine luminal content of IGF-I and IGF-II in uterine flushings from pigs on day 12 of pregnancy were two andthree times greater, respectively, compared with uterine flushings collected from gilts during the oestrous cycle. Both IGF-I and -II content decreased on day 15 of gestation but content of IGF-II in uterine flushings remained three times greater than that of cyclic gilts. IGFBP-2 and -3 were the predominant binding proteins present in uterine flushings during days 0-10 of the oestrous cycle or day 10 of pregnancy. No IGFBPs were detected in theuterine flushings of either cyclic or pregnant pigs after day 10 by ligandblotting. Incubation of [I-125]-labelled IGFBPs with various protease inhibitors indicated that cleavage of [I-125]-labelled IGFBP-2 and -3 in uterine flushings involved serine proteases such as tissue kallikrein and matrix metalloproteinases. The results of the present study indicate that an increase in tissue kallikrein activity on day 12 of the oestrous cycle and pregnancy in pigs can directly, or indirectly through activation of matrix metalloproteinases, cleave IGFBP-2 and -3, thus allowing uterine release of IGF-I and -II in the uterine lumen to stimulate conceptus development.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 08:36:00