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Titolo:
Homologous-pairing activity of the human DNA-repair proteins Xrcc3 center dot Rad51C
Autore:
Kurumizaka, H; Ikawa, S; Nakada, M; Eda, K; Kagawa, W; Takata, M; Takeda, S; Yokoyama, S; Shibata, T;
Indirizzi:
RIKEN, Genom Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan RIKENYokohama Kanagawa Japan 2300045 , Yokohama, Kanagawa 2300045, Japan RIKEN, Mol & Cellular Biol Lab, Wako, Saitama 3510198, Japan RIKEN Wako Saitama Japan 3510198 r Biol Lab, Wako, Saitama 3510198, Japan RIKEN, Harima Inst SPring 8, Cellular Signaling Lab, Sayo, Hyogo 6795143, Japan RIKEN Sayo Hyogo Japan 6795143 Signaling Lab, Sayo, Hyogo 6795143, Japan Univ Tokyo, Japan Sci & Technol Corp, JST, Bunkyo Ku, Tokyo 1130033, JapanUniv Tokyo Tokyo Japan 1130033 orp, JST, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Biochem, Bunkyo Ku, Tokyo 1130033, Japan Kyoto Univ, Fac Med, Sakyo Ku, Kyoto 6068501, Japan Kyoto Univ Kyoto Japan 6068501 , Fac Med, Sakyo Ku, Kyoto 6068501, Japan
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 10, volume: 98, anno: 2001,
pagine: 5538 - 5543
SICI:
0027-8424(20010508)98:10<5538:HAOTHD>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
STRAND BREAK REPAIR; HUMAN RAD51 PROTEIN; COLI RECA PROTEIN; SACCHAROMYCES-CEREVISIAE; ESCHERICHIA-COLI; MAMMALIAN-CELLS; SUPERHELICAL DNA; VERTEBRATE CELLS; RECOMBINATION PROTEIN; GENETIC-RECOMBINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Yokoyama, S RIKEN, Genom Sci Ctr, Tsurumi Ku, 1-7-22 Suehiro Cho, Yokohama, Kanagawa 2300045, Japan RIKEN 1-7-22 Suehiro Cho Yokohama Kanagawa Japan 2300045 Japan
Citazione:
H. Kurumizaka et al., "Homologous-pairing activity of the human DNA-repair proteins Xrcc3 center dot Rad51C", P NAS US, 98(10), 2001, pp. 5538-5543

Abstract

The human Xrcc3 protein is involved in the repair of damaged DNA through homologous recombination, in which homologous pairing is a key step. The Rad51 protein is believed to be the only protein factor that promotes homologous pairing in recombinational DNA repair in mitotic cells. In the brain, however. Rad51 expression is extremely low, whereas XRCC3, a human homologue of Saccharomyces cerevisiae RAD57 that activates the Rad51-dependent homologous pairing with the yeast Rad55 protein, is expressed. In this study, a two-hybrid analysis conducted with the use of a human brain cDNA library revealed that the major Xrcc3-interacting protein is a Rad51 paralog, Rad51C/Rad51L2. The purified Xrcc3 Rad51C complex, which shows apparent 1:1 stoichiometry, was found to catalyze the homologous pairing. Although the activityis reduced, the Rad51C protein alone also catalyzed homologous pairing, suggesting that Rad51C is a catalytic subunit for homologous pairing. The DNA-binding activity of Xrcc3Rad51C was drastically decreased in the absence of Xrcc3, indicating that Xrcc3 is important for the DNA binding of Xrcc3 Rad51C. Electron microscopic observations revealed that Xrcc3Rad51C and Rad51C formed similar filamentous structures with circular single-stranded DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 09:34:31