Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Molecular detection of respiratory syncytial virus in postmortem lung tissue samples from Mexican children deceased with pneumonia
Autore:
Bustamante-Calvillo, ME; Velazquez, FR; Cabrera-Munoz, L; Torres, J; Gomez-Delgado, A; Moreno, JAE; Munoz-Hernandez, O;
Indirizzi:
Hosp Pediat, Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Unidad Invest Med Enfermedades Infecciosas & Para, Mexico City 06725, DF, Mexico Hosp Pediat Mexico City DF Mexico 06725 ra, Mexico City 06725, DF, Mexico Hosp Especialidades, LCM, Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21,Dept Anat Patol, Mexico City, DF, Mexico Hosp Especialidades Mexico City DF Mexico Patol, Mexico City, DF, Mexico
Titolo Testata:
PEDIATRIC INFECTIOUS DISEASE JOURNAL
fascicolo: 5, volume: 20, anno: 2001,
pagine: 495 - 501
SICI:
0891-3668(200105)20:5<495:MDORSV>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
FRAGILE-X SYNDROME; INFANTS; GENE; INFECTION; FMR-1; DNA;
Keywords:
respiratory syncytial virus; reverse transcription-polymerase chain reaction; lung tissue; pneumonia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Bustamante-Calvillo, ME Hosp Pediat, Inst Mexicano Seguro Social, Ctr Med Nacl Siglo 21, Unidad Invest Med Enfermedades Infecciosas & Para, Av Cuauhtemoc 330,Col Doctores, Mexico City 06725, DF, Mexico Hosp Pediat Av Cuauhtemoc 330,Col Doctores Mexico City DF Mexico 06725
Citazione:
M.E. Bustamante-Calvillo et al., "Molecular detection of respiratory syncytial virus in postmortem lung tissue samples from Mexican children deceased with pneumonia", PEDIAT INF, 20(5), 2001, pp. 495-501

Abstract

Background. Respiratory syncytial virus (RSV) is the major viral cause of severe respiratory infections in children younger than 2 years of age. Nevertheless there are not enough epidemiologic data about the role of RSV as acause of infantile mortality from pneumonia, mainly in young children fromdeveloping countries Aim To determine the frequency of RSV infection in lung tissue samples from Mexican children deceased with pneumonia, by reversetranscription (RT) and PCR. Methods. Postmortem lung tissue samples from 98 children younger than 2 years of age who died of pneumonia during the period of 1989 to 1997 were studied. Paraffin was removed with xylene from 10-mum lung sections, the totalRNA was extracted and complementary DNA was obtained by RT reaction. A nested PCR with the use of oligonucleotides specific for the F glycoprotein gene was developed. Samples negatives for RSV were tested for the absence of polymerase inhibitors and for complementary DNA integrity. Results. Twenty-nine of the 98 (30%) children deceased with pneumonia werepositive for RSV by RT-PCR; 8 were detected from 13 (62%) children with histopathologic diagnosis of viral pneumonia and 21 from 85 (25%) children with histopathologic diagnosis of bacterial pneumonia (P = 0.018). There was no significant difference in RSV infection according to age groups or seasonal pattern. Conclusions. RSV infection is frequent in Mexican children younger than 2 years of age who died of pneumonia. Although RSV was more common in viral pneumonia, mixed infections with RSV and bacterial pneumonia were also common.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/10/20 alle ore 00:05:57