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Titolo:
Differential regulation of gelatinases by transforming growth factor beta-1 in normal equine chondrocytes
Autore:
Thompson, CCM; Clegg, PD; Carter, SD;
Indirizzi:
Univ Liverpool, Fac Vet Sci, Connect Tissue Res Grp, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX , Merseyside, England
Titolo Testata:
OSTEOARTHRITIS AND CARTILAGE
fascicolo: 4, volume: 9, anno: 2001,
pagine: 325 - 331
SICI:
1063-4584(200105)9:4<325:DROGBT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX METALLOPROTEINASE ACTIVITY; ARTICULAR CHONDROCYTES; ALGINATE BEADS; OSTEOARTHRITIC CARTILAGE; MESSENGER-RNA; TISSUE INHIBITORS; PROTEOGLYCAN; EXPRESSION; COLLAGEN; TGF-BETA-1;
Keywords:
transforming growth factor beta-1; chondrocytes; gelatinases; equine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Carter, SD Univ Liverpool, Fac Vet Sci, Dept Vet Pathol, Liverpool L69 3BX, Merseyside, England Univ Liverpool Liverpool Merseyside England L69 3BX e, England
Citazione:
C.C.M. Thompson et al., "Differential regulation of gelatinases by transforming growth factor beta-1 in normal equine chondrocytes", OSTEO CART, 9(4), 2001, pp. 325-331

Abstract

Objective: Cartilage destruction in osteoarthritis (OA) is associated withincreased levels of several matrix metalloproteinases (MMPs), including the gelatinases MMP-2 and MMP-9. While increases in some MMPs may be destructive, up-regulation of others may result from increases in normal tissue turnover. The production of MMP-2 and MMP-9 by the anabolic transforming growth factor beta-1 (TGF-beta1) in normal equine chondrocytes was investigated. Design: Equine chondrocytes from clinically normal femoropatellar joints were maintained in alginate beads. After serum deprivation, cells were exposed to TGF-beta1 at several concentrations for varying times. Activities of MMP-2 and MMP-9 were estimated by gelatin zymography, while mRNA for MMP-2,MMP-9 and collagen type II were detected using reverse transcription-polymerase chain reaction. Results: Stimulation with TGF-beta1 differentially regulated MMP-2 and MMP-9, with strong up-regulation of both MMP-9 mRNA and enzyme. Increases in MMP-9 enzyme were dose-dependent (0-49 h). There is some evidence suggestinga slight reduction in MMP-2 release following stimulation. Collagen type II mRNA was transiently increased following stimulation. Conclusions: The different effects of TGF-beta1 on MMP-2 and MMP-9 production by normal chondrocytes suggests different roles for these enzymes. The increases in both MMP-9 and collagen type II mRNA following stimulation maysuggest a role for MMP-9 in tissue maintenance. Therefore, increased MMP-9may be secondary to, as distinct from a cause of, cartilage damage. (C) 2001 OsteoArthritis Research Society International.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 06:52:56