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Titolo:
Sustained activation of the Raf/MEK/Erk pathway in response to EGF in stable cell lines expressing the Hepatitis C Virus (HCV) core protein
Autore:
Giambartolomei, S; Covone, F; Levrero, M; Balsano, C;
Indirizzi:
Univ Rome La Sapienza, Fdn Andrea Cesalpino, Gene Express Lab, Rome, ItalyUniv Rome La Sapienza Rome Italy salpino, Gene Express Lab, Rome, Italy Univ Aquila, Dept Internal Med, I-67100 Laquila, Italy Univ Aquila Laquila Italy I-67100 t Internal Med, I-67100 Laquila, Italy Univ Cagliari, Dept Internal Med, Cagliari, Italy Univ Cagliari CagliariItaly gliari, Dept Internal Med, Cagliari, Italy
Titolo Testata:
ONCOGENE
fascicolo: 20, volume: 20, anno: 2001,
pagine: 2606 - 2610
SICI:
0950-9232(20010503)20:20<2606:SAOTRP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN HEPATOCELLULAR-CARCINOMA; B VIRUS; TRANSCRIPTION FACTORS; RNA HELICASE; HBX PROTEIN; KINASES; RAS; HEPATOCARCINOGENESIS; INTERACTS; CIRRHOSIS;
Keywords:
HCV; core; Raf; Erk1/2; EGF; HCC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Balsano, C Policlin Umberto I, Med Clin 1, Fdn A Cesalpino, Viale Policlin155, I-001612 Rome, Italy Policlin Umberto I Viale Policlin 155 Rome Italy I-001612 taly
Citazione:
S. Giambartolomei et al., "Sustained activation of the Raf/MEK/Erk pathway in response to EGF in stable cell lines expressing the Hepatitis C Virus (HCV) core protein", ONCOGENE, 20(20), 2001, pp. 2606-2610

Abstract

Chronic hepatitis C virus (HCV) infection is a leading cause of liver cirrhosis and hepatocellular carcinoma (HCC) worldwide. The HCV capside core isa multifunctional protein with regulatory functions that affects transcription and cell growth in vitro and in vivo. Here, we show that both HCV genotype 1a and 3 core proteins activate MEK1 and Erk1/2 MAP kinases and that the costitutive expression of the HCV core results in a high basal activity of Raf1 and MAP/kinase/ kinase, as determined by endogenous Raf1 in vitro kinase assay and immunodetection of hyperphosphorylated Erk1 and Erk2 even after a serum starvation. Moreover, the activation of both Erk1/2 and the downstream transcription factor Elk-l in response to the mitogenic stimulus EGF is significantly prolonged. The sustained response to EGF in cells expressing the HCV core occurs despite a normal induction of the MAP phosphatases MKP regulatory feedback and is likely due to the costitutive activation of Raf-l activity. The ability of HCV core proteins to directly activate theMAP kinase cascade and to prolong its activity in response to mitogenic stimuli may contribute to the neoplastic transformation of HCV infected livercells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:58:41