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Titolo:
HUMAN DESMOCOLLIN-1 (DSC1) IS AN AUTOANTIGEN FOR THE SUBCORNEAL PUSTULAR DERMATOSIS TYPE OF IGA PEMPHIGUS
Autore:
HASHIMOTO T; KIYOKAWA C; MORI O; MIYASATO M; CHIDGEY MAJ; GARROD DR; KOBAYASHI Y; KOMORI K; ISHII K; AMAGAI M; NISHIKAWA T;
Indirizzi:
KURUME UNIV,SCH MED,DEPT DERMATOL,67 ASAHIMACHI KURUME FUKUOKA 830 JAPAN UNIV MANCHESTER,EPITHELIAL MORPHOGENESIS RES GRP MANCHESTER LANCS ENGLAND KEIO UNIV,SCH MED,DEPT DERMATOL TOKYO JAPAN
Titolo Testata:
Journal of investigative dermatology
fascicolo: 2, volume: 109, anno: 1997,
pagine: 127 - 131
SICI:
0022-202X(1997)109:2<127:HD(IAA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
DESMOSOMAL GLYCOPROTEIN; ADHESION MOLECULES; VESICULOPUSTULAR DERMATOSIS; PATHOGENIC AUTOANTIBODIES; EXTRACELLULAR DOMAIN; STRATIFIED EPITHELIA; CYTOPLASMIC DOMAINS; MONOCLONAL-ANTIBODY; CELL-ADHESION; FOLIACEUS;
Keywords:
AUTOIMMUNE BULLOUS DISEASE; DESMOSOME; KERATINOCYTE; MAMMALIAN CELL TRANSFECTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
T. Hashimoto et al., "HUMAN DESMOCOLLIN-1 (DSC1) IS AN AUTOANTIGEN FOR THE SUBCORNEAL PUSTULAR DERMATOSIS TYPE OF IGA PEMPHIGUS", Journal of investigative dermatology, 109(2), 1997, pp. 127-131

Abstract

IgA pemphigus showing IgA anti-keratinocyte cell surface autoantibodies is divided into subcorneal pustular dermatosis (SPD) and intraepidermal neutrophilic IgA dermatosis (IEN) types. We previously showed by immunoblotting that IgA from some IgA pemphigus patients reacted with bovine desmocollins (Dsc), but not human Dsc. To determine the antigenfor IgA pemphigus, we focused on conformation-dependent epitopes of Dsc, because sera of patients with classical pemphigus recognize conformation-sensitive epitopes of desmogleins. We constructed mammalian expression vectors containing the entire coding sequences of human Dsc1, Dsc2, and Dsc3 and transiently transfected them into COS7 cells by lipofection. Immunofluorescence of COS7 cells transfected with single human Dscs showed that IgA antibodies of all six SPD-tppe IgA pemphigus cases reacted with the surface of cells expressing Dsc1, but not with cells expressing Dsc2 or Dsc3. In contrast, none of seven IEN-type IgA pemphigus cases reacted with cells transfected with any Dscs. These results convincingly indicate that human Dsc1 is an autoantigen for SPD-type IgA pemphigus, suggesting the possibility of an important role for Dsc1 in the pathogenesis of this disease. This study shows that a Dsc can be an autoimmune target in human skin disease.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 11:10:47