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Titolo:
As to the clastogenic-, sister-chromatid exchange inducing- and cytotoxic activity of inosine triphosphate in cultures of human peripheral lymphocytes
Autore:
Vormittag, W; Brannath, W;
Indirizzi:
Univ Vienna, Div Rheumatol, Dept Internal Med 3, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Internal Med 3, A-1090 Vienna, Austria Univ Vienna, Inst Med Stat, A-1090 Vienna, Austria Univ Vienna Vienna Austria A-1090 Inst Med Stat, A-1090 Vienna, Austria
Titolo Testata:
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
fascicolo: 1-2, volume: 476, anno: 2001,
pagine: 71 - 81
SICI:
1386-1964(20010509)476:1-2<71:ATTCSE>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN-CELLS; ATAXIA TELANGIECTASIA; SCLERODERMA PATIENTS; POOL IMBALANCES; DNA; CHROMOSOMES; INDUCTION; BROMODEOXYURIDINE; HYPOXANTHINE; THYMIDINE;
Keywords:
inosine triphosphate; structural chromosomal aberration; sister-chromatid exchange; cell cycle kinetics; culture of human peripheral lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Vormittag, W Univ Vienna, Div Rheumatol, Dept Internal Med 3, Waehringer Guertel 18-20,A-1090 Vienna, Austria Univ Vienna Waehringer Guertel 18-20 Vienna Austria A-1090 a
Citazione:
W. Vormittag e W. Brannath, "As to the clastogenic-, sister-chromatid exchange inducing- and cytotoxic activity of inosine triphosphate in cultures of human peripheral lymphocytes", MUT RES-F M, 476(1-2), 2001, pp. 71-81

Abstract

The influence of commercial inosine triphosphate (ITP) on the chromosome aberration rate, the mitotic rate, sister-chromatid exchange (SCE) frequency, and the proportion of first (X1), second (X2) and third (X3) division metaphases was investigated in 72 h cultures of human peripheral lymphocytes. The blood donors had mild inactive arthrosis and a normal health check-up. All cultures of each volunteer were set-up simultaneously. In contrast to a previous report [Arch. Biochem. Biophys. 278 (1990) 238-244], it was demonstrated in two preliminary studies (number of subjects, n = 5 each) that ITP at a final concentration of 100 mu does not induce chromosomal aberrations and, furthermore, that not ITP concentrations higher than 100 muM but ITP doses higher than 3.8 mM prohibit culture growth. Based on these results, cultures with a final ITP concentration of 3.6 mM (max.) and 1.8 mM (max./2) were compared with control cultures (number of subjects n = 10; three males and seven females, mean age x = 57.6 years). Whereas no increase in the chromosomal breakage rate was observed in cultureswith an ITP concentration of 1.8 mM and only a marginally significant one (P = 0.048) for 3.6 mM ITP cultures, a highly significant induction of SCEs, not only at an ITP concentration of 3.6 mM (P < 0.0001) but also at 1.8 mM (P < 0.0001) was seen. The increase in the SCE frequency was not linear, but steeper from 0 to 1.8 mM than from 1.8 to 3.6 mM. Nevertheless, the difference between 1.8 and 3.6 mM cultures was significant (P = 0.027). The distribution of the number of SCEs per metaphase as well as the distribution of SCEs per chromosome correspond to the expected Poisson values. The investigation of the cytotoxic effect of the studied ITP concentrations revealeda highly significant reduction of the mitotic rate from 0 to 1.8 mM as well as from 1.8 to 3.6 mM in the aberration studies tall P values are equal to smallest possible one for a sample size of 10, namely, 0.002), and in theSCE studies there is a significant decrease in the X3 frequency when ITP is increased (0-1.8 mM: P = 0.0061 and 1.8-3.6 mM: P < 0.0001). The proportion of X1 within all X1 and X2 metaphases changes significantly only at the second dose step (0-1.8 mM ITP: P = 0.22 and 1.8-3.6 mM ITP: P < 0.0001). The results are discussed. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 19/09/20 alle ore 20:11:11