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Titolo:
BIOAVAILABILITY AND SPECIATION OF MINERAL MICRONUTRIENTS - THE ENZYMOLYSIS APPROACH
Autore:
HOCQUELLET P; LHOTELLIER MD;
Indirizzi:
INST EUROPEEN ENVIRONM BORDEAUX,LAB HYG & SANTE,RUE PROF VEZES F-33300 BORDEAUX FRANCE
Titolo Testata:
Journal of AOAC International
fascicolo: 4, volume: 80, anno: 1997,
pagine: 920 - 927
SICI:
1060-3271(1997)80:4<920:BASOMM>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRON AVAILABILITY; DIETARY FIBER; INVITRO; BINDING; MEALS; SYSTEM; ZINC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
P. Hocquellet e M.D. Lhotellier, "BIOAVAILABILITY AND SPECIATION OF MINERAL MICRONUTRIENTS - THE ENZYMOLYSIS APPROACH", Journal of AOAC International, 80(4), 1997, pp. 920-927

Abstract

Speciation analyses are essential to investigate the effects of dietary components on bioavailability of mineral micronutrients, Enzymolysis was used, An in vitro model simulating enzymatic activity in the gastrointestinal tract of monogastric species was developed and used to assess availability of Fe, Cu, Mn, and Zn in some foodstuffs. The solubility of each element in samples was measured by atomic absorption spectrometry after enzymatic treatment, Data are in good agreement with information obtained from earlier, more expensive nutritional surveys or in vivo experiments and, therefore, allow prediction of the tendencyof a particular food to induce mineral deficiency, In addition, ligands responsible for inhibiting intestinal absorption were identified bydetermining the amount of metal released after treatment of the insoluble residue with an appropriate enzyme such as cellulase and phytase,used respectively to study fiber and phytate interactions, Enzymolysis may be useful for optimizing mineral supplementations though its nutritional significance is somewhat limited by the fact that it does nottake into account the dynamic changes in the gastrointestinal tract, Enzymolysis is a prerequisite for further speciation studies of complex systems and in some instances is the only way for specifying physicochemical forms of elements.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 08:12:45