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Titolo:
Functional production of mammalian concentrative nucleoside transporters in Saccharomyces cerevisiae
Autore:
Vickers, MF; Young, JD; Baldwin, SA; Ellison, MJ; Cass, CE;
Indirizzi:
Univ Alberta, Edmonton, AB, Canada Univ Alberta Edmonton AB CanadaUniv Alberta, Edmonton, AB, Canada Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada Cross Canc Inst Edmonton ABCanada T6G 1Z2 , Edmonton, AB T6G 1Z2, Canada Univ Leeds, Sch Biochem & Mol Biol, Leeds, W Yorkshire, England Univ Leeds Leeds W Yorkshire England l Biol, Leeds, W Yorkshire, England
Titolo Testata:
MOLECULAR MEMBRANE BIOLOGY
fascicolo: 1, volume: 18, anno: 2001,
pagine: 73 - 79
SICI:
0968-7688(200101/05)18:1<73:FPOMCN>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURINE-CYTOSINE PERMEASE; YEAST URACIL PERMEASE; UBIQUITIN-PROTEIN LIGASE; PLASMA-MEMBRANE PROTEIN; XENOPUS-LAEVIS OOCYTES; MOLECULAR-CLONING; INTRACELLULAR MEMBRANES; DRUG-RESISTANCE; CELL-LINE; EXPRESSION;
Keywords:
nucleoside transport; S. cerevisiae; heterologous expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
68
Recensione:
Indirizzi per estratti:
Indirizzo: Cass, CE Univ Alberta, Edmonton, AB, Canada Univ Alberta Edmonton AB Canada v Alberta, Edmonton, AB, Canada
Citazione:
M.F. Vickers et al., "Functional production of mammalian concentrative nucleoside transporters in Saccharomyces cerevisiae", MOL MEMBR B, 18(1), 2001, pp. 73-79

Abstract

The transport of nucleosides and nucleobases in the yeast Saccharomyces cerevisiae is reviewed and the use of this organism to study recombinant mammalian concentrative nucleoside transport (CNT) proteins is described. A selection strategy based on the ability of an expressed nucleoside transportercDNA to mediate thymidine uptake by yeast under a selective condition thatdepletes endogenous thymidylate was used to assess the transport capacity of heterologous transporter proteins. The pyrimidine-nucleoside selective concentrative transporters from human (hCNT1) and rat (rCNT1) complemented the imposed thymidylate depletion in S. cerevisiae, as did N-terminally truncated versions of hCNT1 and rCNT1 lacking up to 31 amino acids. Transporter-mediated rescue of S. cerevisiae by both nucleoside transporters was inhibited by cytidine, uridine and adenosine, but not by guanosine or inosine. This work represents the development of a new model system for the functional production of recombinant nucleoside transporters of the CNT family of membrane proteins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 14:11:40