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Titolo:
Expression of Bcl-2 family proteins and spontaneous apoptosis in normal human testis
Autore:
Oldereid, NB; De Angelis, P; Wiger, R; Clausen, OPF;
Indirizzi:
Natl Hosp, Inst Pathol, N-0027 Oslo, Norway Natl Hosp Oslo Norway N-0027Natl Hosp, Inst Pathol, N-0027 Oslo, Norway Natl Hosp, Dept Obstet & Gynecol, N-0027 Oslo, Norway Natl Hosp Oslo Norway N-0027 Dept Obstet & Gynecol, N-0027 Oslo, Norway Natl Inst Publ Hlth, N-0403 Oslo, Norway Natl Inst Publ Hlth Oslo NorwayN-0403 st Publ Hlth, N-0403 Oslo, Norway
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 5, volume: 7, anno: 2001,
pagine: 403 - 408
SICI:
1360-9947(200105)7:5<403:EOBFPA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERM-CELL APOPTOSIS; IN-VITRO; DEATH; BAX;
Keywords:
apoptosis; Bcl-2 family proteins; immunohistochemistry; spermatogenesis; testis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
17
Recensione:
Indirizzi per estratti:
Indirizzo: Clausen, OPF Natl Hosp, Inst Pathol, N-0027 Oslo, Norway Natl Hosp Oslo Norway N-0027 st Pathol, N-0027 Oslo, Norway
Citazione:
N.B. Oldereid et al., "Expression of Bcl-2 family proteins and spontaneous apoptosis in normal human testis", MOL HUM REP, 7(5), 2001, pp. 403-408

Abstract

We investigated the frequency of spontaneous apoptosis and expression of the Bcl-2 family of proteins during normal spermatogenesis in man. Testicular tissue with both normal morphology and DNA content was obtained from necro-donors and fixed in Bouin's solution. A TdT-mediated dUTP end-labelling method (TUNEL) was used for the detection of apoptotic cells. Expression of apoptosis regulatory Bcl-2 family proteins and of p53 and p21(Waf1) was assessed by immunohistochemistry. Germ cell apoptosis was detected in all testes and was mainly seen in primary spermatocytes and spermatids and in a fewspermatogonia. Bcl-2 and Bak were preferentially expressed in the compartments of spermatocytes and differentiating spermatids, while Bcl-x was preferentially expressed in spermatogonia, Bar showed a preferential expression in nuclei of round spermatids, whereas Bad was only seen in the acrosome region of various stages of spermatids, Mcl-1 staining was weak without a particular pattern, whereas expression of Bcl-w, p53 and p21(Waf1) proteins was not detected by immunohistochemistry. The results show that spontaneous apoptosis occurs in all male germ cell compartments in humans. Bcl-2 family proteins are distributed preferentially within distinct germ cell compartments suggesting a specific role for these proteins in the processes of differentiation and maturation during human spermatogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 09:41:19