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Titolo:
P-2Y1 and P-2Y2 receptor-operated Ca2+ signals in primary cultures of cardiac microvascular endothelial cells
Autore:
Moccia, F; Baruffi, S; Spaggiari, S; Coltrini, D; Berra-Romani, R; Signorelli, S; Castelli, L; Taglietti, V; Tanzi, F;
Indirizzi:
Univ Parma, Dept Evolutionary & Funct Biol, I-43100 Parma, Italy Univ Parma Parma Italy I-43100 ionary & Funct Biol, I-43100 Parma, Italy Univ Pavia, Dept Pharmacol & Physiol Sci, I-27100 Pavia, Italy Univ PaviaPavia Italy I-27100 macol & Physiol Sci, I-27100 Pavia, Italy Univ Brescia, Dept Biomed Sci & Biotechnol, Unit Gen Pathol & Immunol, I-25100 Brescia, Italy Univ Brescia Brescia Italy I-25100 hol & Immunol, I-25100 Brescia, Italy
Titolo Testata:
MICROVASCULAR RESEARCH
fascicolo: 3, volume: 61, anno: 2001,
pagine: 240 - 252
SICI:
0026-2862(200105)61:3<240:PAPRCS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE RECEPTORS; INTRACELLULAR CALCIUM; P2Y(1) RECEPTOR; ATP; P-2Y-PURINOCEPTORS; PURINOCEPTORS; AGONIST; PPADS; P-2U-PURINOCEPTORS; LOCALIZATION;
Keywords:
cardiac microvascular endothelial cell; Ca2+; P-2Y1 receptor; P-2Y2 receptor; ATP; UTP; 2MeSATP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Moccia, F Univ Parma, Dept Evolutionary & Funct Biol, I-43100 Parma, ItalyUniv Parma Parma Italy I-43100 unct Biol, I-43100 Parma, Italy
Citazione:
F. Moccia et al., "P-2Y1 and P-2Y2 receptor-operated Ca2+ signals in primary cultures of cardiac microvascular endothelial cells", MICROVASC R, 61(3), 2001, pp. 240-252

Abstract

Intracellular Ca2+ signals elicited by nucleotide agonists were investigated in primary cultures of rat cardiac microvascular endothelial cells usingthe fura-2 technique. UTP increased the intracellular [Ca2+] in 94% of thecells, whereas 2MeSATP was active in 32%. The rank order of potency was ATP = UTP > 2MeSATP and the maximal response to 2MeSATP was lower compared toUTP and ATP. ATP and UTP showed strong homologous and heterologous desensitization. ATP fully inhibited the 2MeSATP response, while UTP abolished 2MeSATP-elicited transients in 25% of cells. 2MeSATP did not desensitize the UTP or ATP response. Adenosine 2 ' ,5 ' -diphosphate inhibited the response to 2MeSATP, while it did not modify the response to ATP and UTP. 2MeSATP was more sensitive to suramin than UTP and ATP. These results indicate that P-2Y1 and P-2Y2 receptors may be coexpressed in CMEC. Nucleotide-induced Ca2 signals lacked a sustained plateau and were almost independent from extracellular Ca2+. ATP and UTP elicited Ca2+ transients longer than 2MeSATP-evoked transients. The kinetics of Ca2+ responses was not affected by bath solution stirring or ectonucleotidase inhibition. Furthermore, the nonhydrolyzable ATP analogue AMP-PNP induced Ca2+ signals similar to those elicited byATP and UTP. These results suggest that the distinct kinetics of nucleotide-evoked Ca2+ responses do not depend on the activity of ectonucleotidases or ATP autocrine stimulation. The possibility that Ca2+ signals with different time courses may modulate different cellular responses is discussed. (C) 2001 Academic Press.

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Documento generato il 30/11/20 alle ore 16:37:06