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Titolo:
A simple, reliable, and fast protocol for thraustochytrid DNA extraction
Autore:
Mo, CQ; Rinkevich, B;
Indirizzi:
Natl Inst Oceanog, Minerva Ctr Marine Invertebrate Immunol & Dev Bio, IL-31080 Haifa, Israel Natl Inst Oceanog Haifa Israel IL-31080 Dev Bio, IL-31080 Haifa, Israel
Titolo Testata:
MARINE BIOTECHNOLOGY
fascicolo: 2, volume: 3, anno: 2001,
pagine: 100 - 102
SICI:
1436-2228(200103/04)3:2<100:ASRAFP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Keywords:
thraustochytrids; DNA extraction; lysis buffer protocol; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Rinkevich, B Natl Inst Oceanog, Minerva Ctr Marine Invertebrate Immunol & Dev Bio, POB 8030, IL-31080 Haifa, Israel Natl Inst Oceanog POB 8030 HaifaIsrael IL-31080 ifa, Israel
Citazione:
C.Q. Mo e B. Rinkevich, "A simple, reliable, and fast protocol for thraustochytrid DNA extraction", MAR BIOTEC, 3(2), 2001, pp. 100-102

Abstract

dDNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB)or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal UNA polymerase chain reaction, restriction enzyme digestions, andamplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.

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Documento generato il 02/10/20 alle ore 01:39:08