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Titolo:
Possible immortalization of Hodgkin and Reed-Sternberg cells: Telomerase expression, lengthening of telomere, and inhibition of apoptosis by NF-KappaB expression
Autore:
Ohshima, K; Sugihara, M; Haraoka, S; Suzumiya, J; Kanda, M; Kawasaki, C; Shimazaki, K; Kikuchi, M;
Indirizzi:
Fukuoka Univ, Sch Med, Dept Pathol, Jonan Ku, Fukuoka 81401, Japan FukuokaUniv Fukuoka Japan 81401 Pathol, Jonan Ku, Fukuoka 81401, Japan
Titolo Testata:
LEUKEMIA & LYMPHOMA
fascicolo: 3-4, volume: 41, anno: 2001,
pagine: 367 -
SICI:
1042-8194(200104)41:3-4<367:PIOHAR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN FIBROBLASTS; DISEASE; ACTIVATION; CANCER; LYMPHOCYTES; CARCINOMAS; COMPONENT; LYMPHOMAS; DEATH; BCL-2;
Keywords:
hTERT; Hodgkin and Reed-Sternberg cells; apoptosis; NF-kappa B; Hodgkin's lymphoma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Ohshima, K Fukuoka Univ, Sch Med, Dept Pathol, Jonan Ku, Nanakuma 7-45-1, Fukuoka 81401, Japan Fukuoka Univ Nanakuma 7-45-1 Fukuoka Japan 81401 81401, Japan
Citazione:
K. Ohshima et al., "Possible immortalization of Hodgkin and Reed-Sternberg cells: Telomerase expression, lengthening of telomere, and inhibition of apoptosis by NF-KappaB expression", LEUK LYMPH, 41(3-4), 2001, pp. 367

Abstract

Telomerase, an enzyme associated with cellular immortality. is expressed on malignant rumor cells. Deregulation of telomerase is thought to facilitate tumorigenesis and cellular immortality by providing cancer cells with unlimited proliferation capacity. Hodgkin and Reed-Sternberg (H&RS) cells are generally considered as neoplastic cells in Hodgkin`s disease (HD), however, such cells are only found in a minority of HD lesions. In addition, H&RS cells with mitotic features are rare and mummified forms are occasionally encountered. There are no available data on the relationship between telomerase activity acid apoptosis in HD. We studied 14 cases with Hodgkin's disease (mixed cellularity type, nine cases: nodular sclerosis type. five cases)to clarify the relationship between telomerase activity and apoptosis using in situ hybridization of human telomerase reverse transcriptase (hTERT). reverse transcriptase-polymerase chain reaction (RT-PCR) of hTERT. using extracted RNA and immunohistochemistry of nuclear factor-kappaB (NF-kappaB), and TdT-mediated dUTP-digoxigenin nick end-labeling (TUNEL) technique for apoptosis. We also analyzed the telomere length, using sorted H&RS cells. TUNEL showed a few apoptotic H&RS cells. but the cells frequently expressed hTERT. as confirmed by ISH and RT-PCR. Lengthening of the telomere of H&RS cells was noted in ten cases. In addition, H&RS cells frequently expressed NF-kappaB, which is known as an inducible transcription factor and inhibitorof apoptosis. Our findings of telomerase activity in H&RS cells indicate that these cells are neoplastic acid are potentially immortal. In addition, NF-kappaB expression on H&RS cells suggests its possibility in inhibition of apoptosis of these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 08:00:44